The Dorsoventral Boundary of the Germinal Zone is a Specialized Niche for the Generation of Cortical Oligodendrocytes during a Restricted Temporal Window.
The Dorsoventral Boundary of the Germinal Zone is a Specialized Niche for the Generation of Cortical Oligodendrocytes during a Restricted Temporal Window.
复制标题
生发区的背腹边界是受限颞窗期间皮质少突胶质细胞生成的专门生态位。
DOI：
10.1093/cercor/bhv141
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Hitoshi S.
中科院分区：
文献类型：
--
作者：
Naruse M;Ishino Y;Kumar A;Ono K;Takebayashi H;Yamaguchi M;Ishizaki Y;Ikenaka K;Hitoshi S.
少突胶质前体细胞(OPC)出现在胚胎晚期的脑内，成熟后成为少突胶质细胞(OLs)，并在出生后的脑内形成髓鞘。最近，有人提出，来自腹侧前脑的早期出生的OPC在出生后被根除，而晚出生的OPC在成年小鼠的大脑皮质中占主导地位。然而，胚胎大脑中自我更新的多潜能神经干细胞产生皮质OL系细胞的能力的内在和外在因素仍然很大程度上是未知的。使用可诱导的Cre/loxP系统永久标记巢蛋白和寡聚2系细胞，我们发现皮质OL系细胞在E16.5到P10之前的一个有限的时间窗口内开始从神经干细胞分化。然后，我们通过电穿孔的方法将一个表达载体导入E15.5报告小鼠大脑的VZ/SVZ区，结果表明，Wnt信号抑制了VZ/SVZ背侧的神经前体细胞对成人大脑皮质OLS的作用。相反，存在于VZ/SVZ背腹侧边界的神经前体细胞在成人皮质产生大量的OL。我们的结果表明，在这一边界上的神经干细胞是唯一专门在皮质中产生髓鞘形成的OL。
Oligodendrocyte precursor cells (OPCs) appear in the late embryonic brain, mature to become oligodendrocytes (OLs), and form myelin in the postnatal brain. Recently, it has been proposed that early-born OPCs derived from the ventral forebrain are eradicated postnatally and that late-born OLs predominate in the cortex of the adult mouse brain. However, intrinsic and extrinsic factors that specify the ability of self-renewing multipotent neural stem cells in the embryonic brain to generate cortical OL-lineage cells remain largely unknown. Using an inducible Cre/loxP system to permanently labelNestin- andOlig2-lineage cells, we identified that cortical OL-lineage cells start differentiating from neural stem cells within a restricted temporal window just prior to E16.5 through P10. We then showed, by means of electroporation of aCreexpression plasmid into the VZ/SVZ of E15.5 reporter mouse brains, that neural precursor cells in the dorsal VZ/SVZ are inhibited by Wnt signaling from contributing to cortical OLs in the adult brain. In contrast, neural precursor cells present in the dorsoventral boundary VZ/SVZ produce a significant amount of OLs in the adult cortex. Our results suggest that neural stem cells at this boundary are uniquely specialized to produce myelin-forming OLs in the cortex.