Surface modifications and cell-materials interactions with anodized Ti
Surface modifications and cell-materials interactions with anodized Ti
复制标题
DOI：
10.1016/j.actbio.2006.12.003
复制
发表时间：
2007-07-01
影响因子：
9.7
通讯作者：
Bandyopadhyay, Amit
中科院分区：
文献类型：
--
作者：
Das, Kakoli;Bose, Susmita;Bandyopadhyay, Amit
本研究的目的是使用人成骨细胞在阳极氧化钛上研究体外细胞-材料相互作用。钛是一种生物惰性材料，因此在植入活体后会被纤维组织包裹，从而与周围组织隔离。在这项工作中，生物活性的二氧化钛层生长在商业纯钛基板上的阳极氧化过程中使用不同的电解质溶液，即H3 PO 4 HF和H2SO 4。这些电解液产生的生物活性的二氧化钛薄膜具有无孔结构，显示出三个不同的表面形态。使用骨前体细胞系（OPC 1），用原样和阳极化表面研究人成骨细胞生长行为3、5和11天。当阳极氧化表面的细胞材料相互作用进行了比较，注意到，每个表面具有不同的表面特性，这导致细胞材料相互作用的变化。在HF阳极氧化表面观察到细胞定植，并具有独特的细胞间附着。对于用H3 PO 4电解质阳极化的样品，注意到细胞之间具有细胞外基质延伸的良好细胞粘附。在H2SO 4电解质中生长的TiO 2层在表面上没有显示出显著的细胞生长，并且还注意到一些细胞死亡。粘着斑蛋白和碱性磷酸酶，分别在阳极氧化表面上的细胞粘附和分化更明显。3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四唑鎓测定也显示阳极化表面的活细胞密度和增殖增加。很明显，粗糙的表面形态，高的表面能和低的接触角值是更好的细胞材料相互作用的重要因素。在离子浓度与人血浆几乎相同的模拟体液中进行矿化研究，以进一步了解仿生磷灰石沉积行为。与细胞-材料相互作用类似，用不同电解质生长的膜也注意到矿物沉积行为的变化。(c)2007 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
The objective of this study was to investigate in vitro cell-materials interactions using human osteoblast cells on anodized titanium. Titanium is a bioinert material and therefore becomes encapsulated after implantation into the living body by a fibrous tissue that isolates it from the surrounding tissues. In this work, a bioactive TiO2 layer was grown on commercially pure titanium substrate by an anodization process using different electrolyte solutions, namely H3PO4 HF and H2SO4. These electrolytes produced bioactive TiO2 films with a nonporous structure showing three distinctive surface morphologies. Human osteoblast cell growth behavior was studied with as-received and anodized surfaces using an osteoprecursor cell line (OPC 1) for 3, 5 and I I days. When anodized surfaces were compared for cell-materials interaction, it was noticed that each of the surfaces has different surface properties, which led to variations in cell materials interactions. Colonization of the cells was noticed with a distinctive cell-to-cell attachment in the HF anodized surface. Good cellular adherence with extracellular matrix extensions in between the cells was noticed for samples anodized with H3PO4 electrolyte. The TiO2, layer grown in H2SO4 electrolyte did not show significant cell growth on the surface, and some cell death was also noticed. Cell adhesions and differentiation were more pronounced with vinculin protein and alkaline phosphatase, respectively, on anodized surfaces. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyI tetrazolium assays also showed an increase in living cell density and proliferation with anodized surfaces. It was clear that rough surface morphology, high surface energy and low values of contact angles were important factors for better cell materials interaction. A mineralization study was done in simulated body fluid with ion concentrations nearly identical to those of human blood plasma to further understand biomimetic apatite deposition behavior. Similar to cell-materials interaction, variations in mineral deposition behavior were also noticed for films grown with different electrolytes. (c) 2007 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.