Effect of nitro‐L‐arginine on endothelium‐dependent hyperpolarizations and relaxations of pig coronary arteries.
Effect of nitro‐L‐arginine on endothelium‐dependent hyperpolarizations and relaxations of pig coronary arteries.
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硝基-L-精氨酸对猪冠状动脉内皮依赖性超极化和舒张的影响。
DOI：
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
J. Bény
中科院分区：
文献类型：
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作者：
C. Pacicca;P. von der Weid;J. Bény
1. 内皮依赖性松弛是由一种内皮衍生的松弛因子（EDRF）引起的，即一氧化氮（NO）。我们的目的是测试是否存在一种或几种不同的内皮依赖性放松因子。2. 在猪冠状动脉中，高浓度P物质（SP）和缓激肽（BK）引起的超极化伴舒张。3. 为了研究一氧化氮和前列环素在SP和BK引起的猪冠状动脉内皮依赖性松弛和超极化中的作用，我们在监测平滑肌膜电位和等长张力的同时，用NG -硝基- L -精氨酸（L - NNA）抑制NO的产生，用吲哚美辛抑制前列环素的产生。4. 吲哚美辛对前列腺素F2 α预收缩条的静息等距张力和SP、BK弛豫无影响。5. L - NNA收缩内皮完整的动脉条带，而不改变平滑肌的膜电位。6. 该抑制剂使激肽的浓度响应曲线向右移动0.2 nM SP和20 nM BK，最大弛豫和超极化抑制率为30%。7. 结果表明，在猪冠状动脉中，EDRF （NO）主要控制基底张力，而其他因子在激肽引起的超极化和舒张中起重要作用。
1. Endothelium‐dependent relaxation is caused by an endothelium‐derived relaxing factor (EDRF) identified as nitric oxide (NO). Our objective was to test whether one or several distinct endothelium‐dependent relaxing factors exist. 2. In pig coronary arteries, a hyperpolarization accompanied by the relaxation caused by high concentrations of substance P (SP) and bradykinin (BK). 3. To examine the role played by nitric oxide and prostacyclin in the endothelium‐dependent relaxations and hyperpolarizations caused by SP and BK on pig coronary arterial strips, the production of NO was inhibited by NG‐nitro‐L‐arginine (L‐NNA) and the production of prostacyclin was inhibited by indomethacin, while monitoring smooth muscle membrane potential and isometric tension. 4. Indomethacin had no effect on resting isometric tension nor on SP and BK relaxations of strips precontracted by prostaglandin F2 alpha. 5. L‐NNA contracted arterial strips with intact endothelium, without changing the membrane potential of smooth muscles. 6. The inhibitor shifted to the right the concentration‐response curve of kinins by 0.2 nM SP and 20 nM BK. It inhibited the maximal relaxations and hyperpolarizations by 30%. 7. The results show that, in pig coronary arteries, EDRF (NO) mainly controls the basal tension, whereas other factor(s) play(s) an important role in hyperpolarizations and relaxations caused by the kinins.
超极化有助于大鼠股静脉中乙酰胆碱的内皮依赖性松弛。
DOI：
10.1152/ajpheart.1991.261.4.h1034
发表时间：
1991
期刊：
The American journal of physiology
影响因子：
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作者：
Nagao,T;Vanhoutte,PM
通讯作者：
Vanhoutte,PM
一氧化氮、乙酰胆碱以及犬动脉平滑肌的电学和机械特性。
DOI：
10.1152/ajpheart.1988.255.1.h207
发表时间：
1988
期刊：
The American journal of physiology
影响因子：
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作者：
Komori,K;Lorenz,RR;Vanhoutte,PM
通讯作者：
Vanhoutte,PM
内皮源性舒张因子的性质：是否有两种舒张介质？
影响因子：
20.1
作者：
Rubanyi,GM;Vanhoutte,PM
通讯作者：
Vanhoutte,PM