QUANTITATIVE REPRESENTATION OF ALL T-CELLS COMMITTED TO DEVELOP INTO CYTO-TOXIC EFFECTOR-CELLS AND OR INTERLEUKIN-2 ACTIVITY-PRODUCING HELPER-CELLS WITHIN MURINE LYMPHOCYTE-T SUBSETS
QUANTITATIVE REPRESENTATION OF ALL T-CELLS COMMITTED TO DEVELOP INTO CYTO-TOXIC EFFECTOR-CELLS AND OR INTERLEUKIN-2 ACTIVITY-PRODUCING HELPER-CELLS WITHIN MURINE LYMPHOCYTE-T SUBSETS
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DOI：
10.1002/eji.1830140107
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发表时间：
1984-01-01
影响因子：
5.4
通讯作者：
WAGNER, H
中科院分区：
文献类型：
--
作者：
PFIZENMAIER, K;SCHEURICH, P;WAGNER, H
以豆豆蛋白A （cona）刺激为基础的限制性稀释培养系统，研究了lyt -2定义的小鼠T细胞亚群中辅助细胞和细胞毒性前体细胞的定量分布。几乎所有选择的Lyt-2+和lyt - T细胞在8至9天的培养期内生长并扩增成大型克隆菌落。经Con A刺激后，90%的Lyt-2- T细胞能够产生白细胞介素2 （IL -2）活性。8-10%的Lyt-2+ T细胞产生IL2活性。在克隆水平上，Lyt-2+ T细胞产生的IL -2活性的平均值为。与Lyt-2- T细胞相比减少了8倍。细胞毒性T细胞的前体几乎只存在于Lyt-2+群体中，其中。70%的小鼠在凝集素依赖性细胞溶解试验中显示裂解活性。对于绝大多数被分析的克隆，产生IL - 2活性的能力和表达裂解活性的能力是相互排斥的。少数克隆（< 3%）同时产生IL - 2活性并表达细胞毒性。因此，后一种细胞被认为是双功能T细胞。
A limiting dilution culture system based on stimulation with concanavalin A (Con A) was used to study the quantitative distribution of helper and of cytotoxic precursor cells in a Lyt-2-defined subpopulation of murine T cells. Virtually all of the selected Lyt-2+ and Lyt-2- T cells grow and expand to large clonal colonies within an 8 to 9 day culture period. Upon stimulation with Con A, 90% of the Lyt-2- T cells were capable of producing interleukin 2 (IL 2) activity. IL2 activity is produced by 8-10% of Lyt-2+ T cells. At the clonal level, the average of the IL 2 activity produced by Lyt-2+ T cells is .apprx. 8-fold less as compared to Lyt-2- T cells. Precursors of cytotoxic T cells were almost exclusively found in the Lyt-2+ population, of which .apprx. 70% displayed lytic activity in a lectin-dependent cytolysis test. For the vast majority of clones analyzed, the capacity to produce IL 2 activity and the capacity to express lytic activity was mutually exclusive. A minority of clones (< 3%) simultaneously produced IL 2 activity and expressed cytotoxicity. These latter cells are therefore considered as bifunctional T cells.