Centlein, a novel microtubule-associated protein stabilizing microtubules and involved in neurite formation
Centlein, a novel microtubule-associated protein stabilizing microtubules and involved in neurite formation
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Centlein，一种新型微管相关蛋白，可稳定微管并参与神经突形成
DOI：
10.1016/j.bbrc.2016.02.079
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发表时间：
2016-04-01
影响因子：
3.1
通讯作者：
Yuan, Li
中科院分区：
文献类型：
--
作者：
Jing, Zhenli;Yin, Huilong;Yuan, Li
我们之前已经报道了中心粒蛋白centlein作为C-Nap1和Cep68之间的分子连接来维持中心体凝聚力[11]。在这项研究中，我们确定中心肽作为一种新的微管相关蛋白（MAP），直接结合纯化的微管（MT）通过其最长的卷曲螺旋结构域。中心肽的过度表达引起了深刻的诺考达唑和耐寒MT束，这也依赖于其MT结合结构域。siRNA介导的中心肽缺失导致微管蛋白乙酰化水平和细胞质MT乙酰化的总体荧光强度显著降低。中心肽在神经元中进一步表征。我们发现，centlein过表达抑制视黄酸（RA）诱导的SH-SY5Y和N2a细胞的神经突起形成。两者合计，我们建议，中枢肽参与MT的稳定性和轴突在体内。(C)2016 Elsevier Inc.我们以前曾报道过中心粒蛋白centlein作为C-Nap1和Cep68之间的分子连接，以维持中心体的凝聚力[1]。在这项研究中，我们确定中心肽作为一种新的微管相关蛋白（MAP），直接结合纯化的微管（MT）通过其最长的卷曲螺旋结构域。中心肽的过度表达引起了深刻的诺考达唑和耐寒MT束，这也依赖于其MT结合结构域。siRNA介导的中心肽缺失导致微管蛋白乙酰化水平和细胞质MT乙酰化的总体荧光强度显著降低。中心肽在神经元中进一步表征。我们发现，centlein过表达抑制视黄酸（RA）诱导的SH-SY5Y和N2a细胞的神经突起形成。两者合计，我们建议，中枢肽参与MT的稳定性和轴突在体内。(C)2016 Elsevier Inc. All rights reserved.
We have previously reported that the centriolar protein centlein functions as a molecular link between C-Nap1 and Cep68 to maintain centrosome cohesion [11. In this study, we identified centlein as a novel microtubule-associated protein (MAP), directly binding to purified microtubules (MTs) via its longest coiled-coil domain. Overexpression of centlein caused profound nocodazole- and cold-resistant MT bundles, which also relied on its MT-binding domain. siRNA-mediated centlein depletion resulted in a significant reduction in tubulin acetylation level and overall fluorescence intensity of cytoplasmic MT acetylation. Centlein was further characterized in neurons. We found that centlein overexpression inhibited neurite formation in retinoic acid (RA)-induced SH-SY5Y and N2a cells. Taken together, we propose that centlein is involved in MT stability and neuritogenesis in vivo. (C) 2016 Elsevier Inc. All rights reserved.We have previously reported that the centriolar protein centlein functions as a molecular link between C-Nap1 and Cep68 to maintain centrosome cohesion [1]. In this study, we identified centlein as a novel microtubule-associated protein (MAP), directly binding to purified microtubules (MTs) via its longest coiled-coil domain. Overexpression of centlein caused profound nocodazole- and cold-resistant MT bundles, which also relied on its MT-binding domain. siRNA-mediated centlein depletion resulted in a significant reduction in tubulin acetylation level and overall fluorescence intensity of cytoplasmic MT acetylation. Centlein was further characterized in neurons. We found that centlein overexpression inhibited neurite formation in retinoic acid (RA)-induced SH-SY5Y and N2a cells. Taken together, we propose that centlein is involved in MT stability and neuritogenesis in vivo. (C) 2016 Elsevier Inc. All rights reserved.