Suppression of the gene encoding PDZ domain-containing protein decreases cold tolerance and overwintering survival of the mosquito, Culex pipiens (Culicidae: Diptera)
Suppression of the gene encoding PDZ domain-containing protein decreases cold tolerance and overwintering survival of the mosquito, Culex pipiens (Culicidae: Diptera)
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DOI：
10.1093/jme/tjad059
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发表时间：
2023-05-26
影响因子：
2.1
通讯作者：
Sim，Cheolho
中科院分区：
文献类型：
--
作者：
King，Bryan;Larsen，Mazie;Sim，Cheolho
在滞育蚊子中，耐寒和延长寿命是成功越冬的重要特征。在淡色库蚊中，我们认为含有PDZ结构域的蛋白(PDZ)(突触后密度蛋白[PSD95]、果蝇盘大肿瘤抑制因子[Dlg1]和闭锁小带蛋白[ZO-1])结构域蛋白参与了蚊虫越冬存活的滞育特征。在滞育的成虫早期，pDZ的表达水平显著高于非滞育的成虫。通过RNA干扰抑制编码PDZ的基因，显著减少了滞育雌性成虫早期中肠肌动蛋白的积累。抑制pdz还显着降低了滞育雌虫的存活率，这表明该蛋白在滞育早期保存中肠组织中可能起到关键作用。
In diapausing mosquitoes, cold tolerance and prolonged lifespan are important features that are crucial for overwintering success. In the mosquitoCulex pipiens, we suggest that PDZ domain-containing protein (PDZ) (post synaptic density protein [PSD95],drosophila disc large tumor suppressor [Dlg1], andzonula occludens-1 protein [zo-1]) domain-containing protein is involved with these diapause features for overwintering survival inCulexmosquitoes. The expression level ofpdzwas significantly higher in diapausing adult females in the early stage in comparison to their nondiapausing counterparts. Suppression of the gene that encodes PDZ by RNA interference significantly decreased actin accumulation in the midgut of early-stage adult diapausing females. Inhibition ofpdzalso significantly reduced the survivability of diapausing females which indicates that this protein could play a key role in preserving the midgut tissues during early diapause.