Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles.
Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles.
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DOI：
10.3402/jev.v3.26913
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发表时间：
2014
影响因子：
16
通讯作者：
Théry C
中科院分区：
文献类型：
--
作者：
Lötvall J;Hill AF;Hochberg F;Buzás EI;Di Vizio D;Gardiner C;Gho YS;Kurochkin IV;Mathivanan S;Quesenberry P;Sahoo S;Tahara H;Wauben MH;Witwer KW;Théry C
分泌性膜封闭囊泡，统称为细胞外囊泡（EVs），包括外泌体、外泌体、微囊泡、微颗粒、凋亡小体和其他细胞外囊泡亚群，是生物学和医学中一个非常迅速发展的科学领域。重要的是，目前在技术上很难获得不含非囊泡成分的纯EV组分用于功能研究，因此需要建立这些囊泡分析和EV生物学科学研究报告的指南。在这里，国际细胞外囊泡学会（ISEV）为研究人员提供了一套最小的生化、生物物理和功能标准，这些标准应用于将任何特定的生物货物或功能归因于ev。
Secreted membrane-enclosed vesicles, collectively called extracellular vesicles (EVs), which include exosomes, ectosomes, microvesicles, microparticles, apoptotic bodies and other EV subsets, encompass a very rapidly growing scientific field in biology and medicine. Importantly, it is currently technically challenging to obtain a totally pure EV fraction free from non-vesicular components for functional studies, and therefore there is a need to establish guidelines for analyses of these vesicles and reporting of scientific studies on EV biology. Here, the International Society for Extracellular Vesicles (ISEV) provides researchers with a minimal set of biochemical, biophysical and functional standards that should be used to attribute any specific biological cargo or functions to EVs.