Detection and identification of multiple mycobacterial pathogens by DNA amplification in a single tube.
Detection and identification of multiple mycobacterial pathogens by DNA amplification in a single tube.
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通过单管 DNA 扩增检测和鉴定多种分枝杆菌病原体。
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
D. Cousins
中科院分区：
文献类型：
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作者：
S. Wilton;D. Cousins
16 S rRNA的DNA序列的比较揭示了一个区域，在该区域中，分枝杆菌物种之间存在微小的变异。这些信息被用来建立一个多重扩增系统，可以鉴定分枝杆菌属，然后区分M。avium和M.胞内，两种常见的分枝杆菌以外的结核病。将这些rRNA基因引物与针对M.结核复合菌允许在单个管中检测和鉴定临床上重要的分枝杆菌。1030 bp的扩增产物指示分枝杆菌属，850、372和180 bp的较小片段是M的阳性信号。胞内、M.结核复合体（M. tuberculosis complex）; Avium，分别。
A comparison of the DNA sequence of the 16S rRNA revealed a region in which there was a minor variation between the species of mycobacteria. This information was used to develop a multiplex amplification system that could identify the genus Mycobacterium and then distinguish between M. avium and M. intracellulare, two commonly encountered mycobacteria other than tuberculosis. The combination of these rRNA gene primers together with primers aimed at the MPB70 gene of M. tuberculosis complex organisms permits the detection and identification of clinically significant mycobacteria in a single tube. An amplification product of 1030 bp is indicative of the genus Mycobacterium and smaller fragments of 850, 372, and 180 bp are the positive signals for M. intracellulare, M. tuberculosis complex, and M. avium, respectively.