Schistosoma mansoni: in vitro transformation of cercariae into schistosomula
Schistosoma mansoni: in vitro transformation of cercariae into schistosomula
复制标题
曼氏血吸虫：尾蚴体外转化为血吸虫
作者：
B. Gilbert;M. N. Da Rosa;R. Borojevic;J. Pellegrino
曼氏血吸虫尾蚴转化为血吸虫以前已经实现了通过膜的生物来源。这种转化现在是在没有膜的情况下进行的。与人类或大鼠皮肤脂质接触产生转化的化学刺激，后者的分离导致活性成分在含有磷脂的部分中定位。随后发现，涉及转化的一系列过程是由卵磷脂-水混合物对尾蚴水悬浮液的作用而启动的，用粗卵磷脂-水1:50的比例可以重复地获得90%的活血吸虫产量。根据公认的标准，发现幼虫与正常渗透程序产生的幼虫相同。纯化卵磷脂不会产生相同的刺激，并讨论了物理状态和可能存在的杂质在粗卵磷脂中的影响。这项工作得到了Conselho Nacional de Pesquisas（巴西）、Banco Nacional de Desenvolvimento econmico FUNTEC合同第99号和第110号（巴西）、美国陆军补助金DAHC-19-70-G-0017（给B.G.）和DAHC-19-70-G-0070（给J.P.）以及FORGE基金会的支持。没有贡献。18来自巴西米纳斯吉拉斯州联邦大学生物科学研究所血吸虫病研究单位。
The transformation of cercariae of Schistosoma mansoni into schistosomula has previously been achieved by passage through membranes of biological origin. This transformation has now been effected in the absence of a membrane. Chemical stimulus for transformation results from contact with human or rat skin lipid and fractionation of the latter led to localization of the active component in a fraction containing phospholipids. The series of processes involved in transformation was subsequently found to be set in motion by the action of egg lecithin-water mixtures on an aqueous suspension of cercariae and yields of 90% of live schistosomula may be obtained reproducibly with crude egg lecithin-water in the ratio 1:50. The juvenile worms were found, by accepted criteria, to be identical to those produced by the normal penetration procedure. Purified lecithin does not produce the same stimulus and the effect of the physical state and of possible impurities present in crude egg lecithin is discussed. This work was supported by the Conselho Nacional de Pesquisas (Brazil), the Banco Nacional de Desenvolvimento Econâmico FUNTEC contracts nos. 99 and 110 (Brazil), U.S. Army Grants nos. DAHC-19-70-G-0017 (to B.G.) and DAHC-19-70-G-0070 (to J.P.) and by the FORGE Foundation. Contribution no. 18 from the Schistosomiasis Research Unit, Institute of Biological Sciences, Federal University of Minas Gerais, Brazil.