Induction of astroglial gene expression by experimental seizures in the rat: Spatio‐temporal patterns of the early stages
Induction of astroglial gene expression by experimental seizures in the rat: Spatio‐temporal patterns of the early stages
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DOI：
10.1002/(sici)1098-1136(199602)16:2
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发表时间：
1996-02
期刊：
影响因子：
6.2
通讯作者：
N. Belluardo;G. Mudó;X.H. Jiang;D. Condorelli
中科院分区：
文献类型：
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作者：
N. Belluardo;G. Mudó;X.H. Jiang;D. Condorelli
通过原位杂交分析大鼠癫痫持续状态实验模型前 6 小时内胶质纤维酸性蛋白 mRNA 的水平。研究了两种不同的癫痫持续状态模型：一种是通过给锂处理的大鼠施用毛果芸香碱产生的，另一种是通过脑室内给予红藻氨酸产生的。本研究中获得的结果显示，在药物诱导癫痫发作后，GFAP mRNA 水平非常迅速地上调（下丘脑、大脑皮层和海马区的脑室周围区早在 1.5 小时内）。其他几个区域显示 GFAP 从 3 小时开始激活，例如隔膜、缰核、胼胝体和扣带回。对两种癫痫持续状态模型获得的结果进行比较，发现一些大脑区域（例如小脑和纹状体）存在有趣的差异，这可能与药物刺激的特定神经递质受体和神经化学途径有关。有趣的是，一些神经元被毛果芸香碱和红藻氨酸强烈激活的大脑区域（杏仁核和 CA3 海马区）并且经历神经元变性，并没有显示出早期 GFAP 反应。在所检查的几个大脑区域（纹状体、隔膜和下丘脑）中观察到一个有趣的空间特征：反应首先出现在脑室周围区域，然后扩散到大脑区域的其余部分。一般来说，脑室周围区域的 GFAP 反应较早且强烈。 © 1996 Wiley-Liss, Inc.
The levels of glial fibrillary acidic protein mRNA were analysed by in situ hybridization during the first 6 h in experimental models of status epilepticus in the rat. Two different models of status epilepticus were studied: one is produced by the administration of pilocarpine to lithium‐treated rats and the other by the intracerebroventricular administration of kainate. Results obtained in the present study showed a very rapid (as early as 1.5 h in periventricular zones of hypothalamus, cerebral cortex, and hippocampal area) up‐regulation of GFAP mRNA levels following the pharmacological induction of seizures. Several other areas showed a GFAP activation starting at 3 h such as septum, habenular nuclei, corpus callosum, and cingulum. The comparison of the results obtained in the two models of status epilepticus revealed interesting differences in some brain areas, such as cerebellum and striatum, which can be related to the specific neurotransmitter receptors and neurochemical pathways stimulated by the drugs. Interestingly, some brain areas whose neurons are strongly activated by pilocarpine and kainate (amygdala and CA3 hippocampal field) and that undergo neuronal degeneration did not show the early GFAP response. An interesting spatial feature was observed in several brain regions examined (striatum, septum, and hypothalamus): the response first appeared in the periventricular zones and then diffused to the rest of the brain area. In general GFAP responses in the periventricular zones were early and intense. © 1996 Wiley‐Liss, Inc.