Cell cycle-dependent variation of a CD133 epitope in human embryonic stem cell, colon cancer, and melanoma cell lines.
Cell cycle-dependent variation of a CD133 epitope in human embryonic stem cell, colon cancer, and melanoma cell lines.
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人类胚胎干细胞，结肠癌和黑色素瘤细胞系中CD133表位的细胞周期依赖性变化。
DOI：
10.1158/0008-5472.can-08-0723
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发表时间：
2008-10-01
期刊：
影响因子：
11.2
通讯作者：
Oshima, Robert G.
中科院分区：
文献类型：
--
作者：
Jaksch, Marie;Munera, Jorge;Bajpai, Ruchi;Terskikh, Alexey;Oshima, Robert G.
CD133是一种在多种干细胞群和癌症中表达的五跨膜糖蛋白。抗体（AC133）对CD133的糖基化形式的反应性已被广泛用于在xenograph移植试验中富集具有肿瘤启动活性的细胞。我们通过荧光激活的细胞分选发现，在人类胚胎干细胞、结肠癌和黑色素瘤细胞中，AC133反应性的增加与DNA含量的增加相关，并且相互作用，反应性最低的细胞是在细胞周期的G1/G0部分。在AC133反应性高低的基础上继续培养细胞，细胞反应性曲线趋于正常化，这表明低AC133反应性的细胞在恢复增殖时可以产生高反应性的细胞。AC133与活跃循环细胞的关联可能是肿瘤启动活性富集的基础。
CD133 (Prominin1) is a pentaspan transmembrane glycoprotein expressed in several stem cell populations and cancers. Reactivity with an antibody (AC133) to a glycoslyated form of CD133 has been widely used for the enrichment of cells with tumor initiating activity in xenograph transplantation assays. We have found by fluorescence-activated cell sorting that increased AC133 reactivity in human embryonic stem cells, colon cancer and melanoma cells is correlated with increased DNA content and reciprocally, that the least reactive cells are in the G1/G0 portion of the cell cycle. Continued cultivation of cells sorted on the basis of high and low AC133 reactivity results in a normalization of the cell reactivity profiles indicating that cells with low AC133 reactivity can generate highly reactive cells as they resume proliferation. The association of AC133 with actively cycling cells may contribute to the basis for enrichment for tumor initiating activity.