Role of Sox2 and Oct4 in predicting survival of hepatocellular carcinoma patients after hepatectomy
Role of Sox2 and Oct4 in predicting survival of hepatocellular carcinoma patients after hepatectomy
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DOI：
10.1016/j.clinbiochem.2011.02.012
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发表时间：
2011-06-01
影响因子：
2.8
通讯作者：
Yuan, Yunfei
中科院分区：
文献类型：
--
作者：
Huang, Pinzhu;Qiu, Jiliang;Yuan, Yunfei
目的：本研究旨在探讨Sox2和Oct4A在肝细胞癌（HCC）中的预后强度。设计与方法：通过逆转录-聚合酶链反应，研究了Sox2和Oct4A在5个肝癌细胞系、1个永生化正常肝细胞系、匹配的非肿瘤肝组织和正常肝组织中的表达。结果：Sox2和Oct4A mRNA在肝癌细胞系和肿瘤组织中过表达。Sox2或Oct4A阳性表达与侵袭性表型显著相关。单因素和多因素分析均显示Sox2或Oct4A是HCC的独立预后因素。在亚组分析中，Sox2/Oct4A共表达的患者预后最差。进一步分析表明，单独使用Sox2或Sox2/Oct4A可对早期HCC患者的预后进行分层。结论：Sox2和Oct4A可作为肝癌切除术患者预后不良的新预测因子。(C) 2011加拿大临床化学学会。Elsevier Inc.出版。版权所有。
Objectives: The present study was aimed to explore the prognostic strength of Sox2 and Oct4A in hepatocellular carcinoma (HCC).Design and methods: We investigated the expression of Sox2 and Oct4A in five hepatoma cell lines, one immortalized normal liver cell line, HCC tissues with matched nontumorous liver tissues and normal liver tissues by reverse transcription-polymerase chain reaction.Results: Sox2 and Oct4A mRNA were overexpressed in hepatoma cell lines and tumor tissues. Sox2 or Oct4A positive expression was significantly associated with an aggressive phenotype. Both univariate and multivariate analyses revealed that Sox2 or Oct4A was an independent prognostic factor for HCC. When using subgroup analysis, the patients with a co-expression of Sox2/Oct4A had the poorest prognosis. Further analysis demonstrated that Sox2 alone or Sox2/Oct4A could stratify outcome in HCC patients with early stage.Conclusions: Sox2 and Oct4A can be novel predictors of poor prognosis for patients undergoing resection of HCC. (C) 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.