Proteomic analysis for testis of Mice exposed to carbon ion radiation
Proteomic analysis for testis of Mice exposed to carbon ion radiation
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碳离子辐射小鼠睾丸的蛋白质组学分析
DOI：
10.1016/j.mrgentox.2013.06.017
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发表时间：
2013-08-15
影响因子：
1.9
通讯作者：
He, Yang
中科院分区：
文献类型：
--
作者：
Li, Hongyan;Zhang, Hong;He, Yang
本文研究了重离子辐射（HIR）对小鼠睾丸的作用机制。用碳离子束（0.5和4Gy）全身照射雄性小鼠，于照射后第7天取睾丸组织进行分析。采用双向凝胶电泳技术研究了睾丸蛋白质表达的变化。使用PDQuest 8.0软件进行斑点检测和匹配。蛋白质量（标准化斑点体积）的三倍以上差异是检测差异表达蛋白质斑点的标准。采用基质辅助激光解吸/电离串联飞行时间质谱（MALDI-TOF-TOF）技术鉴定蛋白质。通过检索国家生物技术信息中心的蛋白质序列数据库，鉴定出9个特异性蛋白质。这些蛋白质与分子伴侣、代谢酶、氧化应激、精子功能和生精细胞增殖有关。HIR降低谷胱甘肽活性，增加睾丸中丙二醛含量。鉴于Pin 1与细胞周期相关，并且增殖受精子发生的影响，我们通过免疫印迹和免疫荧光分析了睾丸组织学变化和Pin 1蛋白表达。多种途径的改变可能与HIR对睾丸的毒性有关。我们的研究结果是必不可少的发展，生物学和病理学的小鼠睾丸后，在空间或放疗HIR的研究。皇冠版权所有（C）2013由爱思唯尔B. V.出版保留所有权利。
This paper investigates the mechanism of action of heavy ion radiation (HIR) on mouse testes. The testes of male mice subjected to whole body irradiation with carbon ion beam (0.5 and 4Gy) were analyzed at 7 days after irradiation. A two-dimensional gel electrophoresis approach was employed to investigate the alteration of protein expression in the testes. Spot detection and matching were performed using the PDQuest 8.0 software. A difference of more than threefold in protein quantity (normalized spot volume) is the standard for detecting differentially expressed protein spots. A total of II differentially expressed proteins were found. Protein identification was performed using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF-TOF). Nine specific proteins were identified by searching the protein sequence database of the National Center for Biotechnology Information. These proteins were found involved in molecular chaperones, metabolic enzymes, oxidative stress, sperm function, and spermatogenic cell proliferation. HIR decreased glutathione activity and increased malondialdehyde content in the testes. Given that Pin1 is related to the cell cycle and that proliferation is affected by spermatogenesis, we analyzed testicular histological changes and Pin1 protein expression through immunoblotting and immunofluorescence. Alterations of multiple pathways may be associated with HIR toxicity to the testes. Our findings are essential for studies on the development, biology, and pathology of Mouse testes after HIR in space or radiotherapy. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.