7 Log Virus Removal in a Simple Functionalized Sand Filter
7 Log Virus Removal in a Simple Functionalized Sand Filter
复制标题
7 简单功能化砂滤器中的日志病毒去除
DOI：
10.1021/acs.est.9b03734
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发表时间：
2019
影响因子：
11.4
通讯作者：
Nguyen, Thanh H.
中科院分区：
文献类型：
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作者：
Samineni, Laxmicharan;Xiong, Boya;Chowdhury, Ratul;Pei, Andrew;Kuehster, Louise;Wang, Huiran;Dickey, Roman;Soto, Paula Espinoza;Massenburg, Lynnicia;Nguyen, Thanh H.
由于粪便污染导致的饮用水病毒污染难以有效检测和治疗，导致全球范围内的频繁爆发。本文的目的是报告的分子机制，前所未有的高病毒去除从一个实际的砂过滤器。砂过滤器功能化使用辣木（MO）种子的水提取物，功能化的砂（f-砂）过滤器，实现了10.7 log 10病毒去除。这些测试是用MS 2噬菌体进行的，MS 2噬菌体是公认的致病性诺如病毒和轮状病毒的替代物。我们研究了这种高去除率的分子机制，因为它对砂滤（全球最常见的水处理技术）具有重要意义。我们的数据表明，f-砂的病毒清除活性是由于几丁质结合蛋白，M的存在。oleiferachitin-binding protein（MoCBP）在f-sand上的表达。标准柱实验得到蛋白质组学分析和分子对接模拟的支持。我们的模拟表明，MoCBP优先结合MS 2衣壳蛋白，表明特定的分子相互作用是负责增强病毒清除。此外，我们简化了制作f砂的过程，并证明了如何使用盐水再生。目前，在不使用需要高能源或化学品消耗的技术的情况下，还没有针对处理粪便污染的饮用水和灌溉水的病毒的挑战的最终解决方案。我们提出功能化砂（f-砂）过滤器作为一种高效，节能，实用的病毒去除技术，适用于发展中国家和发达国家。
Viral contamination of drinking water due to fecal contamination is difficult to detect and treat effectively, leading to frequent outbreaks worldwide. The purpose of this paper is to report on the molecular mechanism for unprecedented high virus removal from a practical sand filter. Sand filters functionalized using a water extract ofMoringa oleifera(MO) seeds, functionalized sand (f-sand) filters, achieved a ∼7 log10virus removal. These tests were conducted with MS2 bacteriophage, a recognized surrogate for pathogenic norovirus and rotavirus. We studied the molecular mechanism of this high removal since it can have important implications for sand filtration, the most common water treatment technology worldwide. Our data reveal that the virus removal activity of f-sand is due to the presence of a chitin-binding protein,M. oleiferachitin-binding protein (MoCBP) on f-sand. Standard column experiments were supported by proteomic analysis and molecular docking simulations. Our simulations show that MoCBP binds preferentially to MS2 capsid proteins demonstrating that specific molecular interactions are responsible for enhanced virus removal. In addition, we simplified the process of making f-sand and evinced how it could be regenerated using saline water. At present, no definitive solution exists for the challenge of treating fecally contaminated drinking and irrigation water for viruses without using technologies that demand high energy or chemical consumption. We propose functionalized sand (f-sand) filters as a highly effective, energy-efficient, and practical technology for virus removal applicable to both developing and developed countries.