Characterization of lipid fatty acids in whole-cell microorganisms using in situ supercritical fluid derivatization/extraction and gas chromatography mass spectrometry
Characterization of lipid fatty acids in whole-cell microorganisms using in situ supercritical fluid derivatization/extraction and gas chromatography mass spectrometry
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DOI：
10.1021/ac9600767
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发表时间：
1996-09-01
影响因子：
7.4
通讯作者：
Voorhees, KJ
中科院分区：
文献类型：
--
作者：
Gharaibeh, AA;Voorhees, KJ
原位超临界流体衍生和萃取被用作使用脂肪酸谱进行细菌分类的样品制备技术。在静态超临界条件下将季铵盐如苯基三甲基氢氧化铵直接添加到提取容器中的冻干全细胞细菌中，导致细菌脂质的皂化和其脂肪酸的衍生化。然后用超临界CO2萃取衍生的脂肪酸甲酯（FAME），并使用GC/MS进行分析，无需额外的处理。Iso和anteiso C15：0和C17：0沿着C18：0在革兰氏阳性菌中占主导地位，而C16：1，C16：0，C18：1，和环丙基cyC 17：0和cyC 19：0在革兰氏阴性菌中是显着的。应用主成分分析FAME GC/MS数据导致革兰氏阳性菌和革兰氏阴性菌之间的区别。同一属的种间也有分化。
In situ supercritical fluid derivatization and extraction was used as a sample preparation technique for the classification of bacteria using fatty acid profiling. Addition of a quaternary ammonium salt such as phenyltrimethylammonium hydroxide under static supercritical conditions directly to lyophilized, whole-cell bacteria in an extraction vessel resulted in the saponification of the bacterial lipids and derivatization of their fatty acids. The derivatized fatty acid methyl esters (FAMEs) were then extracted with supercritical CO2 and analyzed without additional treatment using GC/MS. Iso and anteiso C15:0 and C17:0 along with C18:0 were predominant in Gram-positive bacteria, while C16:1, C16:0, C18:1, and cyclopropyl cyC17:0 and cyC19:0 were significant in Gram-negative bacteria. Application of principal components analysis to the FAME GC/MS data resulted in the differentiation between Gram-positive and Gram-negative type bacteria. Differentiation between species among the same genera was also observed.