Susceptibility testing to second-line drugs and ethambutol by Genotype MTBDRsl and Bactec MGIT 960 comparing with agar proportion method
Susceptibility testing to second-line drugs and ethambutol by Genotype MTBDRsl and Bactec MGIT 960 comparing with agar proportion method
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DOI：
10.1016/j.tube.2012.05.005
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发表时间：
2012-09-01
期刊：
影响因子：
3.2
通讯作者：
Bouza, E.
中科院分区：
文献类型：
--
作者：
Lopez-Roa, P.;Ruiz-Serrano, M. J.;Bouza, E.
耐多药结核病（MDRTB）的发病率正在增加。快速检测二线药物耐药性对于患者管理和结核病的有效控制至关重要。本研究的目的是评估 GenoType MTBDRsl DNA 条带和 Bactec MGIT 960 检测法检测多重耐药临床分离株对二线药物和乙胺丁醇耐药的能力，使用琼脂比例法作为参考技术。从我们的实验室档案中检索了 26 个根据常规药敏试验鉴定为多重耐药的结核分枝杆菌复合分离株（1992-2010）进行评估。使用MGIT 960和GenoType MTBDRsl前瞻性测试这些菌株对二线药物和乙胺丁醇的敏感性。琼脂比例、MGIT 960和GenoType MTBDRsl的周转时间分别为21天、8天和8小时。 MGIT 960 和 GenoType MTBDRsl 的敏感性值分别为乙胺丁醇 (85.7, 28.6%)、阿米卡星 (50, 75%) 和氧氟沙星 (50, 83.3%)。特异性值分别为乙胺丁醇（73.7, 89.5%）、阿米卡星（72.7, 95.5%）和氧氟沙星（100, 100%）。我们的数据表明，这两种方法都有明显的局限性，不能取代传统的药敏试验。应谨慎解释电阻测试结果并使用参考方法进行确认。 (C) 2012 Elsevier Ltd. 保留所有权利。
The incidence of multidrug-resistant tuberculosis (MDRTB) is increasing. Rapid detection of resistance to second-line drugs is essential for patient management and efficient control of tuberculosis. The aim of the present study was to assess the ability of the GenoType MTBDRsl DNA strip and the Bactec MGIT 960 assay to detect resistance to second-line drugs and ethambutol in multidrug-resistant clinical isolates using the agar proportion method as a reference technique.Twenty-six Mycobacterium tuberculosis complex isolates identified as multidrug-resistant on the basis of conventional drug susceptibility testing were retrieved from our laboratory archive (1992-2010) for evaluation. The susceptibility of these strains to second-line drugs and ethambutol was tested prospectively using MGIT 960 and GenoType MTBDRsl.The turnaround time for agar proportion, MGIT 960, and GenoType MTBDRsl were, respectively, 21 days, 8 days, and 8 h. Sensitivity values for MGIT 960 and GenoType MTBDRsl were, respectively, ethambutol (85.7, 28.6%), amikacin (50, 75%), and ofloxacin (50, 83.3%). Specificity values were, respectively, ethambutol (73.7, 89.5%), amikacin (72.7, 95.5%), and ofloxacin (100, 100%).Our data show that both methods have significant limitations and cannot replace conventional drug susceptibility testing. The results of resistance testing should be interpreted with caution and confirmed using the reference method. (C) 2012 Elsevier Ltd. All rights reserved.