Activities and cellular localization of yeast proteases and their inhibitors.
Activities and cellular localization of yeast proteases and their inhibitors.
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酵母蛋白酶及其抑制剂的活性和细胞定位。
DOI：
10.1016/0006-291x(74)90350-7
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发表时间：
1974
影响因子：
3.1
通讯作者：
J. Meyer
中科院分区：
文献类型：
--
作者：
J. Lenney;P. Matile;A. Wiemken;M. Schellenberg;J. Meyer
我们发现酿酒酵母的蛋白酶A、B和C定位于液泡中。这三种蛋白水解酶的相应抑制物存在于液泡外的胞浆中。酵母蛋白水解酶的区隔表明，它们的功能主要是蛋白质的液泡内消化。Yearst细胞在不同的培养条件下生长，发现诱导细胞生化分化或不允许生长的培养条件会导致蛋白水解酶活性的增强。
We have found that proteases A, B and C of Saccharomyces cerevisiae are localized in the vacuole. The corresponding inhibitors of these three proteases are present in the extravacuolar cytosol. The compartmentation of the yeast proteases suggests that their function is primarily the intravacuolar digestion of proteins. Yearst cells were grown under various conlitions, and it was found that culture conditions which either induce biochemical differentiation of cells or which do not allow growth result in the enhancement of proteolytic activities.