Noninvasive method for assessing the human circadian clock using hair follicle cells
Noninvasive method for assessing the human circadian clock using hair follicle cells
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DOI：
10.1073/pnas.1003878107
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发表时间：
2010-08-31
影响因子：
11.1
通讯作者：
Node, Koichi
中科院分区：
文献类型：
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作者：
Akashi, Makoto;Soma, Haruhiko;Node, Koichi
彻底了解生物钟需要对生物钟基因表达进行定性评估。到目前为止，还没有一种简单有效的检测人体生物钟基因表达的方法。这一限制极大地阻碍了我们对人类昼夜节律的理解。在这里，我们报告了一种方便、可靠、微创的方法，用于检测人体时钟基因表达，使用来自头部或下巴的毛囊细胞活检样本。我们发现，毛囊细胞中生物钟基因表达的昼夜节律阶段准确地反映了个体行为节律，表明这种策略适用于评估人类外周生物钟。此外，使用这种方法，我们表明轮班工人在昼夜基因表达节律和生活方式之间存在严重的时间滞后。因此，对毛囊细胞中的生物钟基因表达进行定性评价可能是临床研究人类生物钟的有效方法。
A thorough understanding of the circadian clock requires qualitative evaluation of circadian clock gene expression. Thus far, no simple and effective method for detecting human clock gene expression has become available. This limitation has greatly hampered our understanding of human circadian rhythm. Here we report a convenient, reliable, and less invasive method for detecting human clock gene expression using biopsy samples of hair follicle cells from the head or chin. We show that the circadian phase of clock gene expression in hair follicle cells accurately reflects that of individual behavioral rhythms, demonstrating that this strategy is appropriate for evaluating the human peripheral circadian clock. Furthermore, using this method, we indicate that rotating shift workers suffer from a serious time lag between circadian gene expression rhythms and lifestyle. Qualitative evaluation of clock gene expression in hair follicle cells, therefore, may be an effective approach for studying the human circadian clock in the clinical setting.