Soil aggregate stratification of nematodes and ammonia oxidizers affects nitrification in an acid soil
Soil aggregate stratification of nematodes and ammonia oxidizers affects nitrification in an acid soil
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线虫和氨氧化剂的土壤团聚体分层影响酸性土壤中的硝化作用
DOI：
10.1111/1462-2920.12339
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发表时间：
2014-10-01
影响因子：
5.1
通讯作者：
Sun, Bo
中科院分区：
文献类型：
--
作者：
Jiang, Yuji;Jin, Chen;Sun, Bo
硝化作用在全球氮循环中起着重要作用，而微生物与土壤动物的相互作用对硝化作用有着重要的影响。在酸性土壤上，研究了连续10年施用有机肥后团聚体中线虫和氨氧化菌数量的同步变化对硝化作用的影响。研究了4种施肥条件下3种团聚体中线虫、氨氧化菌和潜在硝化活性的变化。焦磷酸测序数据显示，优势细菌amoA操作分类单位（OTU）与亚硝化螺旋菌属物种有关，而古细菌OTU隶属于亚硝化球菌属和亚硝化菌属物种。PNA与氨氧化细菌（AOB）丰度的相关性比氨氧化古菌（AOA）丰度更强，尽管AOA在酸性土壤中占主导地位。植物寄生虫对AOB丰度有负面影响;然而，食菌动物比植物寄生虫更能刺激AOB丰度，并对PNA贡献更大。团聚体组分对AOA丰度和AOB群落组成有显著影响。土壤总碳含量对AOA群落的丰度和组成有显著影响，而土壤pH主要影响AOB群落的丰度和组成。土壤变量分别解释了AOA和AOB群落组成变异的62.7%和58.1%，线虫变量分别解释了AOA和AOB群落组成变异的11.7%和19.5%。
Nitrification plays a central role in global nitrogen cycle, which is affected by interaction between soil microfauna and microorganisms. The impact of synchronized changes in nematodes and ammonia oxidizers within aggregate fractions on nitrification was investigated in an acid soil under 10-year manure application. Nematodes, ammonia oxidizers and potential nitrification activity (PNA) were examined in three soil aggregate fractions under four fertilization regimes. Pyrosequencing data revealed that the dominant bacterial amoA operational taxonomic units (OTUs) were related to Nitrosospira species, while archaeal OTUs were affiliated with Nitrososphaera and Nitrosotalea species. PNA was more strongly correlated with ammonia-oxidizing bacteria (AOB) abundance than ammonia-oxidizing archaea (AOA) abundance, although AOA were dominant in the acid soil. Plant parasites had a negative effect on AOB abundance; however, bacterivores stimulated AOB abundance and contributed more to PNA than plant parasites. Aggregate fractions exerted significant impacts on AOA abundance and AOB community composition. Total carbon content strongly affected the abundance and composition of AOA community, while soil pH primarily affected that of AOB community. Soil variables explained 62.7% and 58.1% variations, and nematode variables explained 11.7% and 19.5% variations in the AOA and AOB community composition respectively.