Development of cationic colloidal silica-coated magnetic nanospheres for highly selective and rapid enrichment of plasma membrane fractions for proteomics analysis
Development of cationic colloidal silica-coated magnetic nanospheres for highly selective and rapid enrichment of plasma membrane fractions for proteomics analysis
复制标题
开发阳离子胶体二氧化硅涂层磁性纳米球，用于高选择性和快速富集质膜组分以进行蛋白质组学分析
DOI：
10.1042/ba20090187
复制
发表时间：
2009-12-01
影响因子：
2.8
通讯作者：
Liang, Songping
中科院分区：
文献类型：
--
作者：
Li, Xuanwen;Jia, Xueen;Liang, Songping
PM (plasma membrane) proteins play critical roles in many biological processes and are often used as molecular targets for drug discovery. In PM proteome research, fast and highly selective methods for PM preparation are highly desirable for efficient PM protein identification. In the present study, an improved PM isolation technique involving coating intact cells with synthesized cationic silica-coated magnetic nanospheres was developed and applied to the proteomic analysis of the PM from human erythroleukaemia KS62 cells. Western blotting characterization and protein identification of the prepared PM indicated that the PM enrichment method using the prepared magnetic nanospheres is a fast and inexpensive strategy with high specificity. Our results demonstrate the potential of these cationic silica-coated magnetic nanospheres for high-throughput identification of PM proteins from cells.
