Radiation induced DNA double strand breaks are rejoined by ligation and recombination processes
Radiation induced DNA double strand breaks are rejoined by ligation and recombination processes
复制标题
辐射诱导的 DNA 双链断裂通过连接和重组过程重新连接
DOI：
10.1093/nar/9.13.3139
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发表时间：
1981
影响因子：
14.9
通讯作者：
T. Coquerelle
中科院分区：
文献类型：
--
作者：
K. Weibezahn;T. Coquerelle
用中性条件下滤膜洗脱双链DNA的方法，我们已经证明，大多数γ射线诱导的双链断裂（DSB）在哺乳动物和细菌细胞中重新连接。在V79中国仓鼠细胞的G1期和不同的生长条件下，也会发生重新连接。在37 ℃下8分钟内，一半的断裂重新连接。在E.大肠杆菌的反应同样快，并且依赖于DNA连接酶的存在。E.大肠杆菌重组缓慢，这些需要rec+。未重新连接的DSB分布在DNA上，对染色体中的核小体或接头结构没有任何偏好。两种类型的DSB再连接的区别，DNA连接的快速过程和较慢的过程涉及rec功能。
Using the method of filter elution of double stranded DNA under neutral conditions we have shown that most of gamma-ray induced double strand breaks (DSB) are rejoined in both mammalian and bacterial cells. Rejoining also occurs in the G1 phase in V79 Chinese hamster cells and under different growth conditions. Within 8 minutes at 37 C, half the breaks are rejoined. The rejoining in E. coli is equally fast and depends on the presence of DNA ligase. Some of the breaks in E. coli rejoin slowly, and these require rec+. The non-rejoined DSB are distributed over the DNA without any preference for the nucleosomal or the linker structure in the chromosome. Two kinds of DSB rejoining are discriminated, a fast process of DNA ligation and a slower process involving rec functions.