TRPV2-immunoreactive intrinsic neurons in the rat intestine
TRPV2-immunoreactive intrinsic neurons in the rat intestine
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DOI：
10.1016/j.neulet.2004.05.069
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发表时间：
2004-08-12
影响因子：
2.5
通讯作者：
Ohshima, M
中科院分区：
文献类型：
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作者：
Kashiba, H;Uchida, Y;Ohshima, M
瞬时受体电位通道香草蛋白亚家族2 （TRPV2）接受有害热刺激（> - 52°c），并在细髓鞘传入神经元中表达。在一些外周组织中也检测到mRNA和免疫反应性。我们检测了TRPV2在大鼠肠道中的表达。转录聚合酶链反应（RT-PCR）证实了肠道中TRPV2基因的表达。免疫组化观察到肌肠丛中有大量trpv2阳性神经元。部分神经元calbindin D-28K （CaBP）阳性，CaBP存在于内在传入神经元中。在结节神经节神经元（迷走神经传入）中也观察到TRPV2的免疫反应性。这些发现表明，TRPV2不仅在感觉神经节神经元中表达，也在肠神经元中表达，包括初级传入神经元。2004爱思唯尔爱尔兰有限公司版权所有。
Transient receptor potential channel vanilloid subfamily 2 (TRPV2) was shown to receive noxious thermal stimuli (>52degreesC), and to be expressed in fine myelinated afferent neurons. The mRNA and the immunoreactivity have also been detected in several peripheral tissues. We examined the expression of TRPV2 in the rat intestine. An analysis by transcriptase-polymerase chain reaction (RT-PCR) demonstrated TRPV2 gene expression in the intestine. Many TRPV2-positive neurons were observed in the myenteric plexus by immunohistochemistry. Some of these neurons were positive for calbindin D-28K (CaBP), which is present in intrinsic afferent neurons. TRPV2 immunoreactivity was also observed in nodose ganglion neurons (vagal afferents). These findings suggest that TRPV2 is expressed not only in sensory ganglion neurons, but also in enteric neurons, including primary afferent neurons. (C) 2004 Elsevier Ireland Ltd. All rights reserved.