Cationic ferritin and segmental flow through the trabecular meshwork
Cationic ferritin and segmental flow through the trabecular meshwork
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DOI：
10.1167/iovs.04-0800
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发表时间：
2005-01-01
影响因子：
4.4
通讯作者：
Johnson, DH
中科院分区：
文献类型：
--
作者：
Hann, CR;Bahler, CK;Johnson, DH
目的。为了确定示踪剂分子阳离子铁蛋白(CF)的节段性标记是否指示小梁网络中液体流动的优先模式或细胞和细胞外基质性质的差异。未标记的区域可能表示没有液体进入该区域，或灌流时间不足，或该区域的细胞和细胞外基质不同，不能结合CF.方法：对6只全眼(3只正常，3只假性剥脱[PEX])综合征患者行CF灌流30分钟至4小时。切开小梁网的楔形，并立即固定部分楔形。在固定前，将相邻的楔形放置在CF浴中。结果：随着灌流时间的延长，小梁网内的CF值增加。30min时，CF主要标记葡萄膜和角巩膜区域。4小时后，尽管仍有一些孤立的非标记区存在，但纤维已弥漫在整个网状结构中。浸泡在CF浴中的楔形物在所有时间点都显示较少的未标记区域。PEX物质团块在外围的标记比中心更重，这表明这些团块比周围区域的渗透性更差。PEX眼有相似的标记模式。结论：节段性CF标记提示眼球网中存在优先流动区。随着灌流时间的延长，非标记区逐渐减少。对浸浴组织的大部分区域进行的CF标记表明，未标记的区域在细胞的特征上没有不同，而是CF没有到达这些区域。
PURPOSE. To determine whether segmental labeling by the tracer molecule cationic ferritin ( CF) is indicative of preferential patterns of fluid flow in the trabecular meshwork or of differences in cell and extracellular matrix properties. Nonlabeled regions could indicate no fluid entering that area, insufficient perfusion time, or that the cells and extracellular matrix differ in that region and cannot bind CF.METHODS. Six whole eyes ( three normal and three with pseudoexfoliation [PEX]) syndrome were perfused with CF for 30 minutes to 4 hours. Wedges of trabecular meshwork were dissected and some wedges immediately fixed. Adjacent wedges were placed in a CF bath before fixation. Transmission electron microscopy was used to analyze CF labeling.RESULTS. CF increased in the trabecular meshwork with increasing perfusion time. At 30 minutes, CF labeled mainly the uveal and corneoscleral regions. By 4 hours, CF was found diffusely through the meshwork, although a few isolated nonlabeled areas were still present. Wedges immersed in the CF bath showed fewer nonlabeled regions at all time points. Clumps of PEX material labeled more heavily in the periphery than the center, suggesting the clumps were less permeable than surrounding regions. PEX eyes otherwise had similar labeling patterns.CONCLUSIONS. Segmental labeling with CF implies regions of preferential flow exist in the meshwork. With increasing perfusion time, there were fewer nonlabeled regions. CF labeling of most regions of bath-immersed tissue suggests that nonlabeled regions do not differ in the characteristics of the cells, but rather that CF does not reach these regions.