Genome-Wide Analysis of Small Secreted Cysteine-Rich Proteins Identifies Candidate Effector Proteins Potentially Involved in Fusarium graminearum-Wheat Interactions
Genome-Wide Analysis of Small Secreted Cysteine-Rich Proteins Identifies Candidate Effector Proteins Potentially Involved in Fusarium graminearum-Wheat Interactions
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DOI：
10.1094/phyto-09-15-0215-r
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发表时间：
2016-02-01
期刊：
影响因子：
3.2
通讯作者：
Edwards, Michael C.
中科院分区：
文献类型：
--
作者：
Lu, Shunwen;Edwards, Michael C.
病原体衍生的富含半胱氨酸的小分泌蛋白（SSCP）已知是在特定宿主植物中触发抗性或易感性的真菌效应物的常见来源。这组蛋白质尚未在禾谷镰刀菌中得到很好的研究，禾谷镰刀菌是小麦赤霉病（FHB）的主要原因，小麦赤霉病是一种毁灭性的疾病。我们在这里报告的SSCP编码的基因组中的这种真菌和候选效应蛋白的选择，通过蛋白质组学和序列/转录分析的全面分析。在F.禾谷镰刀菌（分离物PH-1）基于N-末端信号肽序列的存在，成熟蛋白的大小（= 2%）。25（约13%）的SSCP被证实是真正的细胞外蛋白的纳米级液相色谱-串联质谱（nanoLC-MS/MS）分析的最小培养基为基础的体外分泌。序列分析表明，17个SSCP具有保守的功能结构域，包括两个同源的Ecp 2，一个已知的效应产生的番茄病原体叶霉病。转录分析显示，至少有34个SSCP（包括23个在体外分泌组中检测到的）在感染的小麦头中表达;约一半上调与FHB的发展相关的表达模式。这项工作提供了一个坚实的候选人名单，SSCP衍生的效应器，可能发挥作用，在调解F。禾谷-小麦互作本文提出的基于分泌组的体外方法也可用于鉴定作物其他子囊菌病原体中的候选效应子。
Pathogen-derived, small secreted cysteine-rich proteins (SSCPs) are known to be a common source of fungal effectors that trigger resistance or susceptibility in specific host plants. This group of proteins has not been well studied in Fusarium graminearum, the primary cause of Fusarium head blight (FHB), a devastating disease of wheat. We report here a comprehensive analysis of SSCPs encoded in the genome of this fungus and selection of candidate effector proteins through proteomics and sequence/transcriptional analyses. A total of 190 SSCPs were identified in the genome of F. graminearum (isolate PH-1) based on the presence of N-terminal signal peptide sequences, size (= 2%) of the mature proteins. Twenty-five (approximately 13%) SSCPs were confirmed to be true extracellular proteins by nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) analysis of a minimal medium-based in vitro secretome. Sequence analysis suggested that 17 SSCPs harbor conserved functional domains, including two homologous to Ecp2, a known effector produced by the tomato pathogen Cladosporium fulvum. Transcriptional analysis revealed that at least 34 SSCPs (including 23 detected in the in vitro secretome) are expressed in infected wheat heads; about half are up-regulated with expression patterns correlating with the development of FHB. This work provides a solid candidate list for SSCP-derived effectors that may play roles in mediating F. graminearum-wheat interactions. The in vitro secretome-based method presented here also may be applicable for identifying candidate effectors in other ascomycete pathogens of crop plants.