Ultra-high-purity Iron Is a Novel and Very Compatible Biomaterial
Ultra-high-purity Iron Is a Novel and Very Compatible Biomaterial
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超高纯铁是一种新型且相容性非常好的生物材料
DOI：
10.1016/j.jmbbm.2020.103744
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Abiko K
中科院分区：
文献类型：
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作者：
Khan L;Sato K;Okuyama S;Kobayashi T;Ohashi K;Hirasaka K;Nikawa T;Takada K;Higashitani A;Abiko K
金属和合金广泛应用于骨修复材料、支架和牙组织重建。最常见的材料是不锈钢和钴铬镍和钛合金。这些合金很容易变形，但很难断裂。然而，它们对细胞和组织的亲和力非常低。此外，它们有时会引起意想不到的金属过敏。铁是人体必需的一种丰富的微量元素。然而，过量的特别是Fe 2+离子是有毒的。我们以前成功地获得了99.9996%的超高纯铁（ABIKO铁）。ABIKO铁的化学性质与传统纯铁完全不同。例如，在盐酸中的反应速率非常慢，几乎没有任何腐蚀。在这里，我们发现，在没有任何类型的涂层的情况下，哺乳动物细胞可以很容易地附着在ABIKO铁上，并在其上正常增殖和分化。另一方面，由Co-Cr-Mo或Ti-6Al-4V制成的板的表面的细胞密度和增殖率显著降低。此外，几个应激和铁反应基因，HSP 70，SOD 1，ATM和IRP 2在ABIKO铁上没有改变，而这些基因被外源性FeSO 4诱导。细胞还分泌并固定在ABIKO铁上的一些有机物。体外胶原结合试验表明，ABIKO铁结合更高量的胶原。这些发现突出了ABIKO铁作为一种新型生物相容性假体材料。
Metals and alloys are used widely in bone prosthetic materials, stents and dental tissue reconstructions. The most common materials are stainless steels and cobalt-chromium-nickel and titanium alloys. These alloys can be easily deformed but are hard to break. However, their affinity for cells and tissues is very low. In addition, they can sometimes provoke unexpected metal allergies. Iron is an abundant trace element essential for humans. However, excess amounts in particular of Fe2+ions are toxic. We previously succeeded in obtaining 99.9996% ultra-high-purity iron (ABIKO iron). The chemical properties of ABIKO iron are completely different from that of conventional pure iron. For example, the reaction rate in hydrochloric acid is very slow and there is barely any corrosion. Here, we found that, in the absence of any type of coating, mammalian cells could easily attach to, and normally proliferate and differentiate on, ABIKO iron. On the other hand, cell densities and proliferation rate of the surfaces of plates made from Co–Cr–Mo or Ti–6Al–4V were significantly reduced. In addition, several stress and iron response genes, HSP70, SOD1, ATM and IRP2 did not change in the cells on ABIKO iron, while these genes were induced with exogenous application of FeSO4. Cells also secreted and fastened some organics on ABIKO iron. In vitro collagen binding assay showed that ABIKO iron binds higher amount of collagens. These findings highlight ABIKO iron as a novel biocompatible prosthetic material.