A bifunctionalized NiCo2O4-Au composite: Intrinsic peroxidase and oxidase catalytic activities for killing bacteria and disinfecting wound
A bifunctionalized NiCo2O4-Au composite: Intrinsic peroxidase and oxidase catalytic activities for killing bacteria and disinfecting wound
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DOI：
10.1016/j.hazmat.2020.123939
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发表时间：
2021-01-15
影响因子：
13.6
通讯作者：
Cai, Aijun
中科院分区：
文献类型：
--
作者：
Mu, Qianzhu;Sun, Yanfeng;Cai, Aijun
采用焙烧-还原法制备了NiCo 2 O 4-Au复合材料，证明其具有较高的过氧化物酶和类氧化酶活性。该复合物可激活低水平的H2 O2（100 μ M）以杀死E. coli和革兰氏阳性菌S.金黄色。NiCo 2 O 4-Au复合物可通过外加磁铁与介质分离并重复使用。NiCo 2 O 4-Au复合材料也能有效破坏原有生物膜，阻止新生物膜的形成。电子自旋共振测试表明，NiCo 2 O 4- Au复合物催化H2 O2生成活性氧（ROS），主要包括（OH）-O-中心点、O2（中心点-）和O-1（2）;而NiCo 2 O 4-Au复合物的类氧化酶活性也来源于H2 O2不存在时ROS的生成。自由基捕获实验证实，在H2 O2存在下，O中心点H和O-1（2）是NiCo 2 O 4-Au抗菌过程中的主要自由基。设计了一种NiCo 2 O 4-Au基创可贴，该创可贴具有良好的抗感染和促进伤口愈合的性能。本研究表明NiCo 2 O 4-Au复合材料在环境和临床应用中具有良好的应用前景。
A NiCo2O4-Au composite was fabricated following the calcination-reduction method and testified to exhibit the intrinsic high peroxidaseand oxidase-like activities. The composite could activate a low level of H2O2 (100 mu M) to kill E. coli and S. aureus. NiCo2O4-Au composite could be easily separated by an external magnet from the media and reused several times. NiCo2O4-Au composite could also effectively damage the existing biofilms and prevent the formation of new biofilms. The electron spin resonance tests showed that NiCo2O4- Au composite catalyzed H2O2 into reactive oxygen species (ROS), mainly including (OH)-O-center dot, O2(center dot-), and O-1(2); while the oxidase-like activity of NiCo2O4-Au also stemmed from the ROS formation in the absence of H2O2. The radical trapping experiment confirmed that O center dot H and O-1(2) were the main radicals in the antibacterial process for NiCo2O4-Au in the presence of H2O2. A NiCo2O4-Au based Band-Aid was also designed, which exhibited high anti-infective and wound-healing properties. This study has demonstrated that NiCo2O4-Au composite can be a promising anti-bacterial agent in environmental and clinical applications.