ENERGY-METABOLISM OF HUMAN-NEUTROPHILS DURING PHAGOCYTOSIS
ENERGY-METABOLISM OF HUMAN-NEUTROPHILS DURING PHAGOCYTOSIS
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DOI：
10.1172/jci110647
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发表时间：
1982-01-01
影响因子：
15.9
通讯作者：
HERLIN, T
中科院分区：
文献类型：
--
作者：
BORREGAARD, N;HERLIN, T
通过休息和吞噬人类中性粒细胞来产生和利用能量，进行了详细的定量研究。 ATP 含量为 1.9 fmol/细胞，在休息期间保持恒定，并且不受培养基中是否存在葡萄糖的影响。细胞内磷酸肌酸含量<0.2 fmol/细胞。在存在葡萄糖的情况下，ATP 几乎完全由从周围介质中吸收的葡萄糖产生的乳酸产生。产生的乳酸量可以占细胞吸收的葡萄糖的85%，并且细胞内的糖基储存，糖原，没有被利用。根据乳酸产生速率计算的 ATP 产生速率为 1.3 fmol/细胞每分钟。在吞噬作用过程中，葡萄糖消耗或乳酸产生没有明显增加，并且 ATP 含量迅速下降至 0.8 fmol/细胞。 ATP 的这种消失显然是不可逆的，因为没有观察到 ADP 或 AMP 的相应增加。这种吞噬作用引起的 ATP 浓度下降显然代表了人类中性粒细胞在葡萄糖存在的情况下利用的所有额外能量。在没有葡萄糖的情况下，静息细胞中 ATP 生成的速率要小得多，为每分钟 0.75 fmol/细胞，根据糖酵解速率计算得出，糖酵解完全由糖原分解维持。然而，在此条件下，吞噬作用诱导糖原分解显着增强，乳酸生成率增加 60%，将 ATP 生成率提高至每分钟 1.2 fmol/细胞。 ATP 含量从 1.9 fmol/细胞显着下降至 1.0 fmol/细胞。慢性肉芽肿病患者的中性粒细胞与正常细胞具有相同的糖酵解速率和相同的 ATP 含量，从而证实这些细胞的呼吸缺陷不会影响其能量代谢。
Detailed quantitative studies were performed on the generation and utilization of energy by resting and phagocytosing human neutrophils. The ATP content was 1.9 fmol/cell, was constant during rest and was not influenced by the presence or absence of glucose in the medium. The intracellular content of phosphocreatine was < 0.2 fmol/cell. In the presence of glucose, ATP was generated almost exclusively from lactate produced from glucose taken up from the surrounding medium. The amount of lactate produced could account for 85% of the glucose taken up by the cells, and the intracellular glycosyl store, glycogen, was not drawn upon. The rate of ATP generation as calculated from the rate of lactate production was 1.3 fmol/cell per min. During phagocytosis there was no measurable increase in glucose consumption or lactate production, and the ATP content fell rapidly to 0.8 fmol/cell. This disappearance of ATP was apparently irreversible since no corresponding increase in ADP or AMP was observed. This phagocytosis-induced fall in ATP concentration evidently represents all the extra energy utilized in human neutrophils in the presence of glucose. In the absence of glucose, the rate of ATP generation in the resting cell was considerably smaller, 0.75 fmol/cell per min, as calculated from the rate of glycolysis, which was sustained exclusively by glycogenolysis. Under this condition, however, phagocytosis induced significant enhancement of glycogenolysis and the rate of lactate production was increased by 60%, raising the rate of ATP generation to 1.2 fmol/cell per min. The ATP content dropped significantly from 1.9 to 1.0 fmol/cell. Neutrophils from patients with chronic granulomatous disease had the same rate of glycolysis and the same ATP content as normal cells, thus confirming that the defective respiration of these cells did not affect their energy metabolism.