KERATIN-14 PROTEIN IN CULTURED NONPARENCHYMAL RAT HEPATIC EPITHELIAL-CELLS - CHARACTERIZATION OF KERATIN-14 AND KERATIN-19 AS ANTIGENS FOR THE COMMONLY USED MOUSE MONOCLONAL-ANTIBODY OV-6
KERATIN-14 PROTEIN IN CULTURED NONPARENCHYMAL RAT HEPATIC EPITHELIAL-CELLS - CHARACTERIZATION OF KERATIN-14 AND KERATIN-19 AS ANTIGENS FOR THE COMMONLY USED MOUSE MONOCLONAL-ANTIBODY OV-6
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DOI：
10.1002/mc.2940070110
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发表时间：
1993-01-01
影响因子：
4.6
通讯作者：
THORGEIRSSON, SS
中科院分区：
文献类型：
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作者：
BISGAARD, HC;PARMELEE, DC;THORGEIRSSON, SS
我们最近报道了从大鼠肝脏和胰腺分离的非实质来源的细胞系，这些细胞系被认为是体内兼性干细胞隔室的后代，表达一种不寻常的角蛋白（K）组合。这些细胞系表达K8和K14，但不表达K18和K5，它们在细丝形成中的正常伴侣（Bisgaard HC，Thorgeirsson SS，J Cell Physiol 147：333-343，1991）。然而，在自发转化和向成肝细胞样后代分化时，K14表达被废除并被K18的表达取代（Wirth et al.，Electrophoresis 13：305-332，1992）。在本研究中，我们通过蛋白质序列分析证实，K14是肝源性非实质细胞系中中间丝的主要成分。单层细胞的免疫细胞化学分析表明，K8以及K14被纳入细胞骨架。进一步的免疫沉淀分析表明，细丝复合物之间形成的K8和K14作为非典型的合作伙伴。因此，我们得出结论，在一些非实质细胞系分离大鼠肝脏，K8和K14形成一个主要的中间丝网络。最后，我们表明，广泛用于肝和胰腺组织及其肿瘤的细胞系的研究中的抗体，小鼠单克隆抗体OV-6，识别K14和K19中的共同表位。
We have recently reported that cell lines of nonparenchymal origin isolated from rat liver and pancreas, which have been suggested to be the progeny of a facultative stem cell compartment in vivo, express an unusual combination of keratins (K). These cell lines express K8 and K14 but not K18 and K5, their normal partners in filament formation (Bisgaard HC, Thorgeirsson SS, J Cell Physiol 147:333-343, 1991). However, upon spontaneous transformation and differentiation toward a hepatoblastlike progeny, K14 expression is abrogated and replaced by expression of K18 (Wirth et al., Electrophoresis 13:305-332, 1992). In the study presented here, we confirmed by protein sequence analysis that K14 was a major component of the intermediate filaments in a nonparenchymal cell line of hepatic origin. Immunocytochemical analysis of the cells in monolayer demonstrated that K8 as well as K14 were incorporated in the cellular cytoskeleton. Further analysis by immunoprecipitation showed that filament complexes were formed between K8 and K14 as atypical partners. Thus, we concluded that in some nonparenchymal cell lines isolated from rat liver, K8 and K14 form a major intermediate filament network. Finally, we showed that an antibody widely used in studies of the cell lineages of hepatic and pancreatic tissues and their neoplasms, the mouse monoclonal antibody OV-6, recognizes a common epitope in K14 and K19.