Drug resistance testing provides evidence of the globalization of HIV type 1:: A new circulating recombinant form
Drug resistance testing provides evidence of the globalization of HIV type 1:: A new circulating recombinant form
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DOI：
10.1089/0889222041725172
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发表时间：
2004-08-01
影响因子：
1.5
通讯作者：
Salomón, H
中科院分区：
文献类型：
--
作者：
Gómez-Carrillo, M;Quarleri, JF;Salomón, H
为了监测阿根廷HIV-1的多样性，2001年至2003年在布宜诺斯艾利斯市对587名治疗失败患者进行了HIV-1部分pol序列的耐药性检测。从血浆中提取HIV-1 RNA，用RT-PCR扩增pol部分片段。通过自动测序获得序列。进行系统发育分析和重组模式的特点。其中299个序列属于进化枝B（50.94%），284个序列为B/F重组子（48.38%）。4个序列被分为A、C和F分支（0.68%）。发现进化枝C样品96105是BC重组体，并且样品103396和104575显示与先前描述为A2 D重组体的来自肯尼亚的Kisii 5009和来自韩国的97 KR 004相同的嵌合模式。由于存在两个全长基因组，一个来自肯尼亚，一个来自韩国，现在还有两个来自阿根廷的部分基因组，这个重组体被命名为CRF16_A2D。它在三大洲的存在表明CRF16_A2D具有全球分布。
To monitor HIV-1 diversity in Argentina, a phylogenetic-based analysis of HIV-1 partial pol sequences obtained for resistance testing in 587 treatment failure patients was performed in Buenos Aires city between 2001 and 2003. HIV-1 RNA was isolated from plasma samples and partial pol fragments amplified by RTPCR. Sequences were obtained by automated sequencing. Phylogenetic analysis was performed and recombination patterns characterized. A total of 299 sequences grouped into clade B (50.94%) and 284 were B/F recombinants (48.38%). Four sequences were grouped into clades A, C, and F (0.68%). The clade C sample, 96105, was found to be a BC recombinant and samples 103396 and 104575 showed the same mosaic pattern with Kisii5009 from Kenya and 97KR004 from Korea, previously described as A2D recombinants. With the presence of two full-length genomes, one from Kenya and one from Korea, and now two partial genomes from Argentina, this recombinant is designated CRF16_A2D. Its presence on three continents shows that CRF16_A2D has a global distribution.