Interaction of PRMT1 with BTG/TOB proteins in cell signalling:: molecular analysis and functional aspects
Interaction of PRMT1 with BTG/TOB proteins in cell signalling:: molecular analysis and functional aspects
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DOI：
10.1046/j.1356-9597.2001.00497.x
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发表时间：
2002-01-01
期刊：
影响因子：
2.1
通讯作者：
Rouault, JP
中科院分区：
文献类型：
--
作者：
Berthet, C;Guéhenneux, F;Rouault, JP
背景：最近的一些报道将蛋白质甲基化与分化联系起来。此外，BTG/TOB蛋白也参与了这种控制。BTG1和2已被证明与PRMT1（主要的I型细胞精氨酸n -甲基转移酶）相互作用。结果：首先，我们研究了PRMT1与BTG/TOB家族蛋白的相互作用。我们发现boxC，一个只存在于BTG1和BTG2中的序列，对于这种关联是必不可少的。利用boxC肽，我们研究了PRMT1/BTG蛋白在I型蛋白甲基化反应中的重要性。最后，我们发现将boxC融合到穿透素中会干扰PC12细胞和ES细胞衍生的神经元的分化。结论：综上所述，这些结果表明PRMT1/BTG蛋白可能在精氨酸甲基化介导的信号通路和神经元分化中发挥关键作用。
Background: Several recent reports have connected protein methylation with differentiation. Furthermore, the BTG/TOB proteins have also been implicated in such control. BTG1 and 2 have been shown to interact with PRMT1 (predominant cellular arginine N-methyltransferase of type I).Results: First, we have studied the interaction between PRMT1 and the proteins of the BTG/TOB family. We show that boxC, a sequence present only in BTG1 and BTG2, is essential for this association. Using boxC peptide, we have investigated the importance of PRMT1/BTG protein association during type I protein methylation reactions. Finally, we show that the addition of boxC fused to penetratin interferes with the neuronal differentiation of PC12 cells and ES cell-derived neurones.Conclusions: Taken together, these results indicate that PRMT1/BTG proteins could play a key role in the arginine methylation-mediated signalling pathway as well as in neuronal differentiation.