Live cell imaging reveals plant Aurora kinase has dual roles during mitosis
Live cell imaging reveals plant Aurora kinase has dual roles during mitosis
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DOI：
10.1093/pcp/pcn098
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发表时间：
2008-08-01
影响因子：
4.9
通讯作者：
Fukui, Kiichi
中科院分区：
文献类型：
--
作者：
Kurihara, Daisuke;Matsunaga, Sachihiro;Fukui, Kiichi
在有丝分裂过程中，染色体的正确分离是两个子细胞精确分配遗传信息所必需的。在这里，我们使用活细胞成像的微管和动粒处理后的极光激酶抑制剂，橙皮苷，在烟草BY-2细胞分析植物极光激酶在有丝分裂过程中的功能。橙皮苷处理诱导CenH 3在纺锤体赤道上的排列延迟。此外，两种类型的动态滞后CenH 3的染色体分离过程中观察到。研究结果表明，植物极光激酶有双重作用，纠正异常的kinetochoremicrotubule附件和解离的粘连蛋白在染色体排列和分离。
The proper segregation of chromosomes during mitosis is required for accurate distribution of genetic information by two daughter cells. Here, we used live cell imaging of microtubules and kinetochores after treatment with an Aurora kinase inhibitor, hesperadin, in tobacco BY-2 cells to analyze the function of plant Aurora kinase during mitosis. Hesperadin treatment induced the delay of CenH3 alignment on the spindle equator. Furthermore, two types of dynamics of lagging CenH3s were observed during chromosome segregation. The findings indicate that the plant Aurora kinase has dual roles; correction of aberrant kinetochoremicrotubule attachment and dissociation of cohesin during chromosome alignment and segregation.