Binding of calcitonin and calcitonin gene-related peptide to calvarial cells and renal cortical membranes.
Binding of calcitonin and calcitonin gene-related peptide to calvarial cells and renal cortical membranes.
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降钙素和降钙素基因相关肽与颅盖细胞和肾皮质膜的结合。
DOI：
10.1002/jbmr.5650010109
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Cooper,CW
中科院分区：
文献类型：
--
作者：
Seitz,PK;Thomas,ML;Cooper,CW
检查了降钙素（CT）和降钙素基因相关肽（CGRP）与大鼠半钙化和肾膜的结合，以确定CT和CGRP是否与相同的骨细胞结合位点相互作用，并观察骨和肾皮质的结合模式是否相似。未标记的鲑鱼、猪或人CT可抑制125 I-鲑鱼CT与大鼠颅骨的特异性结合，但大鼠CGRP不抑制。CGRP和高剂量鲑鱼CT可抑制125 I-大鼠CGRP与颅骨的结合，但人或猪CT不抑制。未标记的鲑鱼CT或大鼠CGRP可抑制125 I-鲑鱼CT与肾膜的结合，但未检测到125 I-大鼠CGRP的特异性结合。结果提示，骨细胞中存在CT和CGRP的不同受体，CGRP可与肾CT受体相互作用。
Binding of calcitonin (CT) and calcitonin gene‐related peptide (CGRP) to rat hemicalvariae and renal membranes was examined in an effort to determine whether CT and CGRP interact with the same bone cell binding site, and to see whether the binding pattern was similar for bone and renal cortex. Specific binding of125I‐salmon CT to rat calvariae was inhibited by unlabeled salmon, porcine, or human CT, but not by rat CGRP. Binding of125I‐rat CGRP to calvariae was inhibited by CGRP and high doses of salmon CT, but not by human or porcine CT. Binding of125I‐salmon CT to renal membranes was inhibited by unlabeled salmon CT or rat CGRP, but no specific binding of125I‐rat CGRP could be detected. The results suggest that separate bone cell receptors for CT and CGRP exist and that CGRP can interact with renal receptors for CT.