The Uterus Sustains Stable Biological Clock during Pregnancy
The Uterus Sustains Stable Biological Clock during Pregnancy
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DOI：
10.1620/tjem.221.287
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发表时间：
2010-08-01
影响因子：
2.2
通讯作者：
Yaegashi, Nobuo
中科院分区：
文献类型：
--
作者：
Akiyama, Shizuko;Ohta, Hidenobu;Yaegashi, Nobuo
据报道，母体昼夜节律信息在胎儿生理和发育中起重要作用。激素和营养主要被研究为从母亲到胎儿的昼夜节律线索。然而，妊娠生殖器官的昼夜节律特性对胎儿的影响还没有得到充分的研究。为了深入了解生殖器官的昼夜节律功能，我们检查了妊娠大鼠子宫和胎盘中的分子钟。通过使用Period 1-荧光素酶（Per 1-luc）大鼠，其组织表达对应于Period 1（一种“关键时钟基因”）激活的荧光素酶，我们在非妊娠期间、胚胎第12天（E12）和E22（妊娠结束）在光暗（LD）周期和恒定黑暗（DD）中检查了子宫时钟。通过原位杂交，我们进一步探讨了E12和E22胎盘中Pen 1 mRNA的节律。子宫在体外表现出明确的昼夜节律Per 1-luc的节奏，在怀孕和不怀孕，有高峰在周围的时间对应于黄昏在LD。同样，在DD中，离体子宫具有相同的Per 1-luc节律。在LD的蜕膜表现出昼夜Per 1 mRNA的节律，在黄昏后6小时达到峰值，而在DD的蜕膜表现出相同的Per 1 mRNA的节律，只有在E22。与此相反，迷宫显示没有昼夜Per 1 mRNA节律在LD或DD在怀孕期间。这些结果表明，子宫和蜕膜，胎盘的母体来源的组织，而不是迷路，胎盘的胎儿来源的组织，可以提供胎儿的昼夜信息。
Maternal circadian information has been reported to play an important role in fetal physiology and development. Hormones and nutrition have been mainly investigated as circadian cues from mother to fetus. However, the influences of circadian properties of the pregnant reproductive organs on fetuses have not been fully investigated. To gain an insight on the circadian functions of the reproductive organs, we examined molecular clocks in the pregnant rat uterus and placenta. By using a Period1-luciferase (Per1-luc) rat, whose tissues express luciferase corresponding to activation of Period1, a "key clock gene", we examined the uterus clock during non-pregnancy, on embryonic day 12 (E12), and on E22 (the end of pregnancy) in a light-dark (LD) cycle and constant darkness (DD). By in situ hybridization we further explored Pen l mRNA rhythms in the placenta on E12 and E22. The uterus in vitro showed clear circadian Per1-luc rhythms both in and out of pregnancy, having peaks at around the time corresponding to dusk in LD. Likewise, in DD, the uterus in vitro had the same Per1-luc rhythms. The decidua in LD showed circadian Per1 mRNA rhythms, peaking during night 6 h after dusk, while the decidua in DD showed the same Per1 mRNA rhythms only on E22. In contrast, the labyrinth showed no circadian Per1 mRNA rhythms in LD or DD during pregnancy. These results suggest that the uterus and decidua, a maternally-originated tissue of the placenta, but not the labyrinth, a fetus-originated tissue of the placenta, can provide the fetus with circadian information.