Identification of rabbit microsomal cytochrome P-450 isozyme, form 1, as a hepatic progesterone 21-hydroxylase.
Identification of rabbit microsomal cytochrome P-450 isozyme, form 1, as a hepatic progesterone 21-hydroxylase.
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兔微粒体细胞色素 P-450 同工酶 1 型的鉴定，为肝孕酮 21-羟化酶。
DOI：
10.1016/0006-291x(82)91465-6
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发表时间：
1982
影响因子：
3.1
通讯作者：
Johnson,EF
中科院分区：
文献类型：
--
作者：
Dieter,HH;Muller-Eberhard,U;Johnson,EF
六个高度纯化形式的兔微粒体细胞色素P-450，分离自肝微粒体，表现出不同的孕酮的区域特异性代谢。只有一种同工酶研究，形式1，催化形成脱氧皮质酮从孕酮在一个可观的速度。这种细胞色素P-450同工酶可能参与妊娠期间孕酮向脱氧皮质酮的转化。所有六种形式的细胞色素P-450在两种供试孕酮浓度下均催化6β-和16α-羟基化。晶型3b对6β-羟基化的表观Km值低于其他五种晶型。
Six highly purified forms of rabbit microsomal cytochrome P-450, isolated from hepatic microsomes, exhibit differences in the regiospecific metabolism of progesterone. Only one of the isozymes studied, form 1, catalyzes the formation of deoxycorticosterone from progesterone at an appreciable rate. This cytochrome P-450 isozyme may participate in the conversion of progesterone to deoxycorticosterone during pregnancy. All six forms of cytochrome P-450 catalyze 6β- and 16α-hydroxylation at the two concentrations of progesterone tested. Form 3b exhibits a lower apparent Km for 6β-hydroxylation than the other five.