Intravesicular administration of sodium hyaluronate ameliorates the inflammation and cell proliferation of cystitis cystica et glandularis involving interleukin-6/JAK2/Stat3 signaling pathway.
Intravesicular administration of sodium hyaluronate ameliorates the inflammation and cell proliferation of cystitis cystica et glandularis involving interleukin-6/JAK2/Stat3 signaling pathway.
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膀胱内给予透明质酸钠改善涉及白细胞介素6/JAK2/Stat3信号通路的囊性和腺性膀胱炎的炎症和细胞增殖
DOI：
10.1038/s41598-017-16088-9
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发表时间：
2017-11-21
影响因子：
4.6
通讯作者：
Shi B
中科院分区：
文献类型：
--
作者：
Ni Y;Zhao S;Yin X;Wang H;Guang Q;Hu G;Yang Y;Jiao S;Shi B
囊性及腺性膀胱炎（CCEG）是一种慢性膀胱炎，会给患者带来极大的痛苦。然而，目前缺乏有效的保守治疗方法。本研究表明，囊内透明质酸钠（SH）治疗明显改善了CCEG患者的临床症状，改善了该疾病的膀胱黏膜炎症和细胞增殖特征。免疫组化染色显示，与对照组相比，CCEG患者膀胱粘膜组织透明质酸酶（HYAL 1/2）、CD44、IL-6和磷酸化信号换能器和转录激活因子3 （p-Stat3）的染色强度显著升高，囊泡内SH治疗抑制了这些蛋白的表达。我们通过大肠杆菌囊内处理大鼠，建立了CCEG大鼠模型，发现大肠杆菌组与NC组相比，HYAL 1/2和CD44的表达水平显著升高。与NC组相比，大肠杆菌组IL-6/JAK2/Stat3通路的激活以及下游促凋亡蛋白Mcl-1和Bcl-xL的表达水平也显著升高。囊泡内SH治疗可显著减轻上述变化。因此，SH可能通过抑制膀胱粘膜炎症和增殖作为CCEG的有效治疗方法。
Cystitis cystica et glandularis (CCEG) is a chronic cystitis that causes extreme agony in affected patients. However, there are lack of effective conservative treatments. In this study, it is evident that intravesicular sodium hyaluronate (SH) therapy significantly improved the clinical symptoms of CCEG patients and ameliorated the bladder mucosal inflammation and cell proliferation characteristics of the disease. Immunohistochemical staining showed that the staining intensities of hyaluronidase (HYAL 1/2), CD44, IL-6 and phosphorylated signal transducer and activator of transcription 3 (p-Stat3) in bladder mucosal tissue were significantly increased in CCEG patients compared with control patients and that intravesicular SH treatment suppressed these protein expression. We established a CCEG rat model by treating rats with E. coli intravesicularly, and we found that HYAL 1/2 and CD44 expression levels were significantly increased in the E. coli group compared with the NC group. Activation of the IL-6/JAK2/Stat3 pathway and the expression levels of the downstream pro-apoptotic proteins Mcl-1 and Bcl-xL were also significantly increased in the E. coli group compared with the NC group. The above changes were significantly mitigated by intravesicular SH treatment. Therefore, SH may serve as an effective therapy for CCEG by inhibiting bladder mucosal inflammation and proliferation.
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影响因子：
50.3
作者：
Bromberg J;Wang TC
通讯作者：
Wang TC
影响因子：
1.8
作者：
Fursov, Natalie;Gates, Irina V.;Powers, Gordon
通讯作者：
Powers, Gordon
影响因子：
1.9
作者：
Li, Aihua;Liu, Sikuan;Zhng, Binghui
通讯作者：
Zhng, Binghui
影响因子：
5.6
作者：
Kunze D;Erdmann K;Froehner M;Wirth MP;Fuessel S
通讯作者：
Fuessel S
影响因子：
2
作者：
Cervigni M
通讯作者：
Cervigni M