Morphine and opioid peptides reduce paraventricular neuronal activity: studies on the rat hypothalamic slice preparation.
Morphine and opioid peptides reduce paraventricular neuronal activity: studies on the rat hypothalamic slice preparation.
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吗啡和阿片肽减少室旁神经元活动：大鼠下丘脑切片制备的研究。
DOI：
10.1073/pnas.77.9.5527
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发表时间：
1980
影响因子：
11.1
通讯作者：
Bloom,FE
中科院分区：
文献类型：
--
作者：
Pittman,QJ;Hatton,JD;Bloom,FE
在大鼠下丘脑脑片上记录到室旁核(PVN)神经元的细胞外放电。根据(I)时相活动模式和(II)来自神经垂体束的逆行侵袭的标准来识别PVN神经元(n=14)。没有显示这两种特征的神经元被认为是生理功能未知的(n=85)。大多数未鉴定的神经元对沐浴应用吗啡(1微米)、脑啡肽(D-Ala2，Met5)或β-内啡肽(0.01-1微米)有反应，表现为迅速、可逆的、剂量相关的放电频率减少。纳洛酮(1微米)在部分但不是全部病例中对抗阿片类药物诱导的抑郁。在测试的浓度下，没有观察到对阿片类药物影响的快速反应。阿片类药物对神经垂体神经元的影响不那么明显；虽然有2个神经元受到抑制，但其余12个神经元对微摩尔浓度的吗啡、[D-Ala2，Met5]脑啡肽或β-内啡肽的放电频率或放电模式没有变化。我们的结果表明，阿片类药物通过与一种特定的阿片受体相互作用抑制大鼠下丘脑室旁核的神经元活动，但这种作用在未鉴定的神经元上比在脑片中假定的神经垂体神经元上更明显。
Extracellular discharges of neurons in the paraventricular nucleus (PVN) were recorded from slices of rat hypothalamus in vitro. PVN neurons (n = 14) were identified by the criteria of (i) phasic activity patterns and (ii) antidromic invasion from the neurohypophysial tract. Neurons not displaying either of these features were considered unidentified with respect to physiological function (n = 85). The majority of unidentified neurons responded to bath application of morphine (1 microM), [D-Ala2,Met5]enkephalin (1 microM) or beta-endorphin (0.01-1 microM) with a prompt, reversible, dose-related reduction in spike discharge frequency. Naloxone (1 microM) antagonized the opioid-induced depressions in some, but not all, cases. At the concentrations tested, no tachyphylaxis to the effects of the opioids was observed. The opioid effects on putative neurohypophysial neurons were less pronounced; while 2 were depressed, the remaining 12 displayed no change in frequency or pattern of discharge to micromolar concentrations of morphine, [D-Ala2,Met5]enkephalin, or beta-endorphin. Our results indicate that opioids depress neuronal activity in the rat PVN via an interaction with a specific opiate receptor but that this effect is more pronounced on unidentified neurons than on putative neurohypophysial neurons in the slice.