3D printed porous Ti6Al4V cage: Effects of additive angle on surface properties and biocompatibility; bone ingrowth in Beagle tibia model
3D printed porous Ti6Al4V cage: Effects of additive angle on surface properties and biocompatibility; bone ingrowth in Beagle tibia model
复制标题
3D打印多孔Ti6Al4V保持架：附加角度对表面性能和生物相容性的影响；
DOI：
10.1016/j.matdes.2019.107824
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发表时间：
2019-08-05
影响因子：
8.4
通讯作者：
Lee, In-Seop
中科院分区：
文献类型：
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作者：
Chen, Cen;Hao, Ya;Lee, In-Seop
与自体骨相似的力学性能、良好的生物相容性和骨整合性是椎间融合器的关键因素。在本研究中，计算机设计的宏观和微观结构的多孔Ti6Al4V融合器复制选择性激光熔化（SLM）。制备了具有六种不同角度的致密Ti6Al4V圆盘，以研究附加角（SLM制造中不可避免的）对表面性能和生物相容性的影响。随着角度的增加，由于未熔化的金属颗粒数量的增加，表面粗糙度和疏水性都增加。在细胞培养的早期阶段，MC3T3E1细胞在加角较大时，细胞的伸长程度更明显，但不同角度的细胞粘附和增殖能力差异无统计学意义。具有开放窗口、孔隙率为70%、孔径为600 μ m的子弹形Ti6Al4V椎间融合器显示，在029%应变下的弹性模量为0.51 +/-0.04 GPa，极限压缩强度为94.7 MPa，与市售聚醚醚酮椎间融合器相当。通过组织学分析对Beagle胫骨模型进行体内评价，结果表明，具有相同孔隙率和孔径的圆柱形多孔体在4周和12周时实现了与市售多孔钽植入物相似的骨长入，表明SLM制造的多孔Ti6Al4V椎间融合器具有很大的脊柱融合潜力。(C)2019作者爱思唯尔有限公司出版
Similar mechanical properties to native bone, excellent biocompatibility and osseointegration are key factors for interbody fusion cage. In the present study, porous Ti6Al4V cage with computer designed macro- and micro-architecture was reproduced by selective laser melting (SLM). Dense Ti6Al4V discs with six different angles were fabricated to investigate the effects of additive angle (inevitable in SLM manufacturing) on surface properties and biocompatibility. As angle increased, both surface roughness and hydrophobicity increased due to increases in the number of unmelted metallic particles. In the early stage of cell culture, more elongated MC3T3E1 cells were observed at higher additive angle, but cell adhesion and proliferation with different angle did not show statistically differences. Bullet-shaped Ti6Al4V cage with opened window, porosity of 70% and pore size of 600 mu m revealed elastic modulus of 0.51 +/- 0.04 GPa and ultimate compressive strength of 94.7 MPa at 029% strain, which is comparable with the commercialized polyetheretherketone cage. In vivo evaluation of Beagle tibia model through histological analysis showed that the cylindrical porous body with same porosity and pore size achieved similar bone ingrowth as commercialized porous tantalum implant at 4 and 12 weeks, indicating SLM fabricated porous Ti6Al4V cage holds great potential for spinal fusion. (C) 2019 The Authors. Published by Elsevier Ltd.