Oral microbiota species in acute apical endodontic abscesses
Oral microbiota species in acute apical endodontic abscesses
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DOI：
10.3402/jom.v8.30989
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发表时间：
2016-01-01
影响因子：
4.5
通讯作者：
Machida, Curtis A.
中科院分区：
文献类型：
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作者：
George, Noelle;Flamiatos, Erin;Machida, Curtis A.
背景和目的：急性根尖脓肿是一种严重的牙髓疾病，由机会性口腔微生物感染牙髓所致。这项研究的目的是确定和比较来自俄勒冈州波特兰人口学区域的急性根尖脓肿患者(N=18)的口腔微生物区系。研究假设是，从这个人口统计地区获得的脓肿可能含有从其他地区的样本中没有鉴定出的独特微生物。设计：牙髓脓肿取自俄勒冈健康与科学大学(OHSU)牙科学院的患者。用16S rRNA基因特异性引物聚合酶链式反应扩增脓肿标本DNA，Cy3-dCTP标记。结果：在多个脓肿标本中发现了最常见的微生物，包括核梭杆菌、微小单胞菌、巨藻克隆CS025、多糖类普雷沃特氏菌、齿状隐杆菌属和牙髓卟啉单胞菌。在单个脓肿中发现的数量最多的微生物包括核杆菌、P.Micra、链球菌簇III、嗜酸单胞菌、星状链球菌和牙髓卟啉单胞菌。多糖类普氏杆菌、链球菌科物种克隆DM071、巨型细菌克隆CS025、放线菌物种克隆EP053和临界链球菌之间有很强的细菌关联性(均具有斯皮尔曼系数>；0.9)。结论：从俄勒冈州波特兰人口统计地区获得的牙髓脓肿中已鉴定出可培养和不可培养细菌种类，分类群鉴定与其他已发表的研究有很好的相关性，除了密螺旋体和甲型链球菌外，这两种细菌在波特兰、俄勒冈和其他地区的人口统计地区牙髓脓肿中并不常见。
Background and objectives: Acute apical abscesses are serious endodontic diseases resulting from pulpal infection with opportunistic oral microorganisms. The objective of this study was to identify and compare the oral microbiota in patients (N = 18) exhibiting acute apical abscesses, originating from the demographic region in Portland, Oregon. The study hypothesis is that abscesses obtained from this demographic region may contain unique microorganisms not identified in specimens from other regions.Design: Endodontic abscesses were sampled from patients at the Oregon Health & Science University (OHSU) School of Dentistry. DNA from abscess specimens was subjected to polymerase chain reaction amplification using 16S rRNA gene-specific primers and Cy3-dCTP labeling. Labeled DNA was then applied to microbial microarrays (280 species) generated by the Human Oral Microbial Identification Microarray Laboratory (Forsyth Institute, Cambridge, MA).Results: The most prevalent microorganisms, found across multiple abscess specimens, include Fusobacterium nucleatum, Parvimonas micra, Megasphaera species clone CS025, Prevotella multisaccharivorax, Atopobium rimae, and Porphyromonas endodontalis. The most abundant microorganisms, found in highest numbers within individual abscesses, include F. nucleatum, P. micra, Streptococcus Cluster III, Solobacterium moorei, Streptococcus constellatus, and Porphyromonas endodontalis. Strong bacterial associations were identified between Prevotella multisaccharivorax, Acidaminococcaceae species clone DM071, Megasphaera species clone CS025, Actinomyces species clone EP053, and Streptococcus cristatus (all with Spearman coefficients > 0.9).Conclusions: Cultivable and uncultivable bacterial species have been identified in endodontic abscesses obtained from the Portland, Oregon demographic region, and taxa identifications correlated well with other published studies, with the exception of Treponema and Streptococcus cristae, which were not commonly identified in endodontic abscesses between the demographic region in Portland, Oregon and other regions.