Genome-wide identification, evolution, and expression analysis of RNA-binding glycine-rich protein family in maize.
Genome-wide identification, evolution, and expression analysis of RNA-binding glycine-rich protein family in maize.
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DOI：
10.1111/jipb.12210
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发表时间：
2014-10
影响因子：
11.4
通讯作者：
Jianhua Zhang;Yanxin Zhao;Hailin Xiao;Yong-lian Zheng;B. Yue
中科院分区：
文献类型：
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作者：
Jianhua Zhang;Yanxin Zhao;Hailin Xiao;Yong-lian Zheng;B. Yue
RNA 结合富含甘氨酸的蛋白 (RB-GRP) 家族的特征是存在以 (Gly)n-X 重复排列的富含甘氨酸的结构域和 RNA 识别基序 (RRM)。 RB-GRPs参与多种生理生化过程，尤其是植物的逆境响应。本研究在玉米（Zea mays L.）中共鉴定出分布在10条染色体上的23个RB-GRP，并根据其保守结构域将它们分为4个亚类。鉴定出五对旁系同源物，但它们都不位于同一染色体区域，这表明节段重复在玉米 RB-GRP 的重复事件中占主导地位。对玉米、拟南芥 (Arabidopsis thaliana L.)、水稻 (Oryza sativa L.) 和小麦 (Triticum aestivum) 中 RB-GRP 的比较分析表明，仅在玉米中发现了两个独特的亚组。八种 ZmRB-GRP 的表达受到至少两种应激的显着调节。此外，ZmRB-GRPs启动子区预测的顺式元件也表明这些ZmRB-GRPs可能参与玉米的逆境响应。对玉米中 RB-GRP 的初步全基因组分析将为进一步研究 ZmRB-GRP 的功能提供有用的信息。
The RNA-binding glycine-rich protein (RB-GRP) family is characterized by the presence of a glycine-rich domain arranged in (Gly)n-X repeats and an RNA-recognition motif (RRM). RB-GRPs participate in varied physiological and biochemical processes especially in the stress response of plants. In this study, a total of 23 RB-GRPs distributed on 10 chromosomes were identified in maize (Zea mays L.), and they were divided into four subgroups according to their conserved domain architecture. Five pairs of paralogs were identified, while none of them was located on the same chromosomal region, suggesting that segmental duplication is predominant in the duplication events of the RB-GRPs in maize. Comparative analysis of RB-GRPs in maize, Arabidopsis (Arabidopsis thaliana L.), rice (Oryza sativa L.), and wheat (Triticum aestivum) revealed that two exclusive subgroups were only identified in maize. Expression of eight ZmRB-GRPs was significantly regulated by at least two kinds of stresses. In addition, cis-elements predicted in the promoter regions of the ZmRB-GRPs also indicated that these ZmRB-GRPs would be involved in stress response of maize. The preliminary genome-wide analysis of the RB-GRPs in maize would provide useful information for further study on the function of the ZmRB-GRPs.