Effects of habitual exercise and dietary restriction on intrahepatic and periepididymal fat accumulation in Zucker fatty rats.
Effects of habitual exercise and dietary restriction on intrahepatic and periepididymal fat accumulation in Zucker fatty rats.
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DOI：
10.1186/s13104-015-1063-6
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发表时间：
2015-04-02
影响因子：
1.8
通讯作者：
Minato K
中科院分区：
文献类型：
--
作者：
Kurosaka Y;Shiroya Y;Yamauchi H;Kaneko T;Okubo Y;Shibuya K;Minato K
习惯性运动和饮食限制通常被推荐用于预防或改善肥胖和与生活方式相关的疾病，包括脂肪肝。本研究探讨了习惯性运动和饮食限制对Zucker fatty（ZF）大鼠肝脏甘油三酯（TG）水平、血清瘦素水平和附睾周围脂肪组织中脂肪细胞大小的影响。将六周龄雄性ZF大鼠随机分配到三组之一：久坐组（Sed）、久坐和饮食限制组（Sed + DR）以及训练和饮食限制组（TR + DR）。雄性Zucker lean（L）大鼠用作对照动物。所有大鼠均可随意饮水和允许数量的食物。Sed + DR组和Tr + DR组大鼠饲喂30%限制性饲料，而Tr + DR组大鼠在轮式测力计上进行自愿运动。12周后，处死大鼠，对其肝脏和附睾周围脂肪组织进行组织学检查。同时测定肝脏和血清TG、血清总胆固醇、谷草转氨酶、谷草转氨酶、游离脂肪酸和瘦素水平。Sed + DR组肝脏TG水平显著高于L组（P < 0.001）和Sed组（P < 0.05）。Tr + DR组肝组织TG水平显著低于Sed组（P < 0.05）和Sed + DR组（P < 0.001），但与L组无显著性差异。Sed组、Sed + DR组和Tr + DR组的附睾周围脂肪细胞显著大于L组（P < 0.001），Tr + DR组显著小于Sed组和Sed + DR组（P < 0.001）。我们的研究结果表明ZF大鼠的脂质代谢和脂肪细胞的大小之间的关系。运动在降低肝脏TG水平、血清瘦素水平和脂肪细胞大小方面起着重要作用。
Habitual exercise and dietary restriction are commonly recommended to prevent or ameliorate obesity and lifestyle-related diseases, including fatty liver. This study investigated the effects of habitual exercise and dietary restriction on hepatic triglyceride (TG) levels, serum leptin levels, and histological adipocyte size in periepididymal adipose tissue from Zucker fatty (ZF) rats. Six-week-old male ZF rats were randomly assigned to one of three groups: sedentary (Sed), sedentary and dietary restriction (Sed + DR), and training and dietary restriction (Tr + DR). Male Zucker lean (L) rats were used as control animals. All rats had access to water and the allowed quantity of food ad libitum. The rats in the Sed + DR and Tr + DR groups were fed a 30% restricted diet, while those in the Tr + DR group exercised voluntarily on a wheel ergometer. After 12 weeks, the rats were sacrificed for a histological examination of their liver and periepididymal adipose tissue. Hepatic and serum TG, serum total cholesterol, glutamic-oxaloacetic transaminase, glutamic-pyruvic transaminase, free fatty acid, and leptin levels were also measured. The hepatic TG levels were significantly higher in the Sed + DR group than in the L (P < 0.001) and Sed (P < 0.05) groups. By contrast, the hepatic TG levels in the Tr + DR group were significantly lower than those in the Sed (P < 0.05) and Sed + DR (P < 0.001) groups, but not significantly different from the L group values. The periepididymal adipocytes were significantly larger in the Sed, Sed + DR, and Tr + DR groups than in the L group (P < 0.001) and were significantly smaller in the Tr + DR group compared to the Sed and Sed + DR groups (P < 0.001). Our results suggest a relationship between lipid metabolism and the size of adipose cells in ZF rats. Exercising plays an important role in decreasing hepatic TG levels, serum leptin levels, and the size of adipose cells.