Microvalves for integrated selective droplet generation, splitting and merging on a chip
Microvalves for integrated selective droplet generation, splitting and merging on a chip
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DOI：
10.1007/s10404-021-02487-y
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发表时间：
2021-11-01
影响因子：
2.8
通讯作者：
Neild, Adrian
中科院分区：
文献类型：
--
作者：
Agnihotri, Sagar N.;Raveshi, Mohammad Reza;Neild, Adrian
在本文中，我们展示了一个选择性的多位点分裂和合并系统进行高通量筛选所需的反应的排列类型。我们的方法使用多个单层微流体阀，因为它们提供简单性，生物相容性和高速操作。这些阀门用于控制液滴的产生和分裂的位置。在前者中，阀用于完全阻塞一些微通道;在后者中，它们产生更适度的局部几何形状变化。生成的液滴充当在芯片周围运输不同样品的容器;然后这些容器可以在分裂位置处被细分，就像移液管可以用于分配其总体积的一部分一样。分裂的液滴进入合并通道，其中不同类型的子液滴可以合并。我们证明了不同的液滴类型在不同的浓度在四个合并位置的合并。
In this paper, we demonstrate a selective multisite splitting and merging system to perform the type of permutations of reactions required for high-throughput screening. Our approach uses multiple single-layer microfluidic valves as they offer simplicity, biocompatibility, and high-speed operation. These valves are used both to control the generation of droplets and the location of splitting. In the former, the valves are used to completely block some microchannels; in the latter, they create a more modest local geometry variation. The generated droplets act as a vessel that transports different samples around the chip; these vessels can then be subdivided at splitting locations, just as a pipette can be used to dispense a fraction of its total volume. The split droplets enter a merging channel, in which daughter droplets of different types can be merged. We demonstrate the merging of different droplet types in different concentrations at four merging locations.