A novel activity of HMG domains: promotion of the triple-stranded complex formation between DNA containing (GGA/TCC)11 and d(GGA)11 oligonucleotides.
A novel activity of HMG domains: promotion of the triple-stranded complex formation between DNA containing (GGA/TCC)11 and d(GGA)11 oligonucleotides.
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HMG 结构域的一项新活性：促进含有 (GGA/TCC)11 和 d(GGA)11 寡核苷酸的 DNA 之间形成三链复合物。
DOI：
10.1093/nar/24.23.4733
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发表时间：
1996
影响因子：
14.9
通讯作者：
R. Kominami
中科院分区：
文献类型：
--
作者：
T. Suda;Y. Mishima;K. Takayanagi;H. Asakura;S. Odani;R. Kominami
高迁移率族蛋白（HMG）-盒是在许多蛋白质中发现的DNA结合结构域，其以不依赖于序列的方式优先结合不规则结构的DNA，并且可以使DNA弯曲。我们在这里表明，GST融合蛋白的HMG域从HMG 1和HMG 2促进三链复合物的形成之间的DNA含有（GGA/TCC）11重复和寡核苷酸的d（GGA）11可能是由于G：G碱基配对。活性是减少缔合时间以及Mg 2+和寡核苷酸浓度的要求。决定雄性性别分化的蛋白质SRY的HMG盒也具有活性，这表明它不限于来自HMG 1/2的HMG盒结构域，而是与来自HMG盒蛋白质家族的其他成员的HMG盒结构域共有。有趣的是，在环化测定中，HMG 1的box-AB和box-B弯曲含有重复序列的DNA，但SRY不能弯曲。这种差异表明，促进结合和DNA弯曲这两种活动是不同的。这些结果表明，HMG-box结构域具有促进GGA重复序列之间结合的新活性，其可能参与染色质的高级结构。
The high mobility group protein (HMG)-box is a DNA-binding domain found in many proteins that bind preferentially to DNA of irregular structures in a sequence-independent manner and can bend the DNA. We show here that GST-fusion proteins of HMG domains from HMG1 and HMG2 promote a triple-stranded complex formation between DNA containing the (GGA/TCC)11 repeat and oligonucleotides of d(GGA)11 probably due to G:G base pairing. The activity is to reduce association time and requirements of Mg2+ and oligonucleotide concentrations. The HMG box of SRY, the protein determining male-sex differentiation, also has the activity, suggesting that it is not restricted to the HMG-box domains derived from HMG1/2 but is common to those from other members of the HMG-box family of proteins. Interestingly, the box-AB and box-B of HMG1 bend DNA containing the repeat, but SRY fails to bend in a circularization assay. The difference suggests that the two activities of association-promotion and DNA bending are distinct. These results suggest that the HMG-box domain has a novel activity of promoting the association between GGA repeats which might be involved in higher-order architecture of chromatin.
DOI：
10.1016/s0021-9258(18)63741-x
发表时间：
1989-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Yvonne Hodges-Garcia;P. Hagerman;D. Pettijohn
通讯作者：
Yvonne Hodges-Garcia;P. Hagerman;D. Pettijohn
影响因子：
56.9
作者：
PIL, PM;LIPPARD, SJ
通讯作者：
LIPPARD, SJ
影响因子：
4.4
作者：
BECKMANN, JS;WEBER, JL
通讯作者：
WEBER, JL