CHANGES IN LIPID CLASS AND FATTY-ACID CONTENTS IN THE OVARY AND MIDGUT GLAND OF THE FEMALE FIDDLER-CRAB UCA TANGERI (DECAPODA, OCYPODIADAE) DURING MATURATION
CHANGES IN LIPID CLASS AND FATTY-ACID CONTENTS IN THE OVARY AND MIDGUT GLAND OF THE FEMALE FIDDLER-CRAB UCA TANGERI (DECAPODA, OCYPODIADAE) DURING MATURATION
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DOI：
10.1007/bf00349488
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发表时间：
1994-12-01
期刊：
影响因子：
2.4
通讯作者：
RODRIGUEZ, A
中科院分区：
文献类型：
--
作者：
MOURENTE, G;MEDINA, A;RODRIGUEZ, A
本文研究了桔海招潮蟹成熟过程中卵巢和中肠腺的生化组成、脂类和脂肪酸含量的变化。1992年春季和初夏，在圣佩德罗河口附近的加迪斯湾（西班牙西南部）捕获了野生雌性。卵巢中的蛋白质和总脂质含量从第III阶段到第IV阶段显著增加，总碳水化合物在同一时期大幅下降。在中肠腺中，蛋白质含量在成熟过程中变化不显著，而总脂肪和总碳水化合物含量则呈相反的上升和下降趋势。在卵巢中，总极性脂质在成熟的最后阶段（阶段III至IV）显着增加，主要是由于磷脂酰胆碱和磷脂酰乙醇胺组分的显着贡献。相反，总中性脂质呈上升趋势，在整个成熟期，主要是由于三酰甘油馏分的显着增加。在中肠腺，总极性脂质（主要是磷脂酰胆碱）和总中性脂质（主要是三酰甘油）提出了显着的下降，从第二阶段到第三阶段，这一阶段之前的极性和中性脂质在卵巢中的主要增加。胆固醇含量没有变化成熟过程中的任何器官，在卵巢或中肠腺。然而，卵巢中的主要脂肪酸[16：0、16：1（n-7）、18.1（n-9）、18：1（n-7）、18：2（n-6）、18：3（n-3）、20：4（n-6）、20：5（n-3）和22：6（n-3）]在成熟后期显著积累。值得注意的是，花生四烯酸[20：4（n-6）]含量在成熟的所有阶段保持不变，但在成熟的后期阶段，总极性脂质显着下降。相反，二十碳五烯酸[20：5（n-3）]在后期的所有脂质组分中显著增加，二十二碳六烯酸[22：6（n-3）]在极性脂质中保持恒定，在后期的三酰甘油组分中增加。中肠腺脂质中的主要脂肪酸表现出显着下降，从第二阶段到第三阶段，就在最后一个时期的成熟。
Changes in biochemical composition, lipid class and fatty acid contents were studied in the ovaries and midgut glands of the fiddler crabs Uca tangeri Eydoux during maturation. Wild females were caught during spring and early summer of 1992 in the Bay of Cadiz (southwest Spain), near the mouth of the San Pedro river. Protein and total lipid contents in the ovaries increased significantly from Stages III to IV, at the expense of total carbohydrate, which showed a large decrease during the same period. In the midgut gland, the protein content did not present any significant variation, whereas total lipids and total carbohydrates presented opposite up and down trends during maturation. In the ovary, total polar lipids increased significantly during the final phase of maturation (Stages III to IV), mainly due to the significant contribution of the phosphatidylcholine and phosphatidylethanolamine fractions. In contrast, total neutral lipids showed an upward trend throughout the whole maturation period, mainly due to significant increases of the triacylglycerol fraction. In the midgut gland, total polar lipids (mainly phosphatidylcholine) and total neutral lipids (mainly triacylglycerol) presented significant decreases from Stages II to III, the phase which preceded major increases in both polar and neutral lipids in the ovaries. Cholesterol content did not vary during maturation in either organ, in the ovary or midgut gland. Major fatty acids in the ovaries [16:0, 16:1 (n-7), 18.1 (n-9), 18:1 (n-7), 18:2 (n-6), 18:3 (n-3), 20:4 (n-6), 20:5 (n-3) and 22:6 (n-3)] did, however, accumulate significantly at later stages of maturation. It is noteworthy that arachidonic acid [20:4 (n-6)] content remained constant during all stages of maturation but decreased significantly in total polar lipids in the later phases of maturation. In contrast, eicosapentaenoic acid [20:5 (n-3)] increased significantly in all lipid fractions in the later stages, and docosahexaenoic acid [22:6 (n-3)] remained constant in the polar lipids and increased during later stages in the triacylglycerol fraction. Major fatty acids in the midgut gland lipids showed significant decreases from Stages II to III, just before the final period of maturation.