Regenerative potentials evoked in circular smooth muscle of the antral region of guinea‐pig stomach
Regenerative potentials evoked in circular smooth muscle of the antral region of guinea‐pig stomach
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DOI：
10.1111/j.1469-7793.1999.0563t.x
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发表时间：
1999-06
期刊：
影响因子：
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通讯作者：
H. Suzuki;G. Hirst
中科院分区：
文献类型：
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作者：
H. Suzuki;G. Hirst
1从豚鼠上颌圆形平滑肌层记录的慢波由两部分组成，一个初始部分和一个二次再生部分。尽管两种成分在硝苯地平的存在下仍然存在，但次要成分被低浓度的咖啡因所消除。2分离单束圆形肌肉的短段，用两个微电极刺穿。去极化电流引起的再生反应类似于正常慢波引起的再生反应。这些反应的部分不应期为20 - 30秒，并在膜去极化开始后约1秒开始。在硝苯地平或钴离子存在下，再生反应持续存在，但被咖啡因、BAPTA或环吡唑酸消除。观察结果表明，去极化的膜电位变化触发Ca2+从细胞内储存的释放，这通过激活豚鼠胃窦圆形层平滑肌细胞膜上的一组未知离子通道导致去极化。
1 Slow waves recorded from the circular smooth muscle layer of guinea‐pig antrum consisted of two components, an initial component and a secondary regenerative component. Whereas both components persisted in the presence of nifedipine, the secondary component was abolished by a low concentration of caffeine. 2 Short segments of single bundles of circular muscle were isolated and impaled with two microelectrodes. Depolarizing currents initiated regenerative responses which resembled those initiated during normal slow waves. These responses had partial refractory periods of 20‐30 s and were initiated about 1 s after the onset of membrane depolarization. 3 The regenerative responses persisted in the presence of either nifedipine or cobalt ions but were abolished by caffeine, BAPTA or cyclopiazonic acid. 4 The observations suggest that depolarizing membrane potential changes trigger the release of Ca2+ from intracellular stores and this causes a depolarization by activating sets of unidentified ion channels in the membranes of smooth muscle cells of the circular layer of guinea‐pig antrum.