SIMPLE METHOD FOR PREPARATION OF SINGLE CELL-SUSPENSIONS FROM NORMAL AND TUMOROUS RAT COLONIC MUCOSA
SIMPLE METHOD FOR PREPARATION OF SINGLE CELL-SUSPENSIONS FROM NORMAL AND TUMOROUS RAT COLONIC MUCOSA
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DOI：
10.1136/gut.18.5.382
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发表时间：
1977-01-01
期刊：
影响因子：
24.5
通讯作者：
PIGMAN, W
中科院分区：
文献类型：
--
作者：
PERRET, V;LEV, R;PIGMAN, W
通过使用含有0.2M甘露醇的磷酸盐缓冲盐水从大鼠结肠上皮获得活的单细胞悬液。该方法不需要预先进行酶处理，可在1小时内以高产率提供未受损的细胞。该程序也适用于肿瘤大鼠结肠组织，这是由重复直肠内输注N-甲基-N-亚硝基脲诱导。正常和肿瘤细胞之间的比较表明，后者具有较高的核质比和较高的代谢活性。
Viable single cell suspensions from rat colonic epithelium were obtained by using phosphate buffered saline containing 0.2 M mannitol. The method, which requires no prior enzyme treatment, provides undamaged cells in high yield within 1 h. The procedure was also applied to neoplastic rat colonic tissue, which was induced by repeated intrarectal infusion of N-methyl-N-nitrosourea. Comparison between normal and neoplastic cells showed that the latter have a higher nucleus: cytoplasm ratio and higher metabolic activity.