Effect of salicylic acid on the antioxidant system and photosystem II in wheat seedlings
Effect of salicylic acid on the antioxidant system and photosystem II in wheat seedlings
复制标题
水杨酸对小麦幼苗抗氧化系统和光系统II的影响
DOI：
10.1007/s10535-015-0564-4
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发表时间：
2016-01-01
影响因子：
1.5
通讯作者：
Zhang, H. Y.
中科院分区：
文献类型：
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作者：
Chen, Y. E.;Cui, J. M.;Zhang, H. Y.
为了研究施用水杨酸 (SA) 对小麦幼苗抗氧化系统和光系统 II (PS II) 的影响，我们使用了两个不同的实验。第一种方法是将根部浸入含有 0、0.25 或 2.5 mM SA 的 Hoagland 营养液中，第二种方法是用相同浓度的 SA 喷洒两周龄的幼苗。 24小时后，测量叶绿素荧光、类囊体膜蛋白、抗氧化酶活性和活性氧。低浓度的SA导致抗氧化酶活性显着增加。然而，2.5 mM SA处理导致非光化学猝灭系数增加，抗氧化酶活性、PS II光化学量子产率和光化学猝灭降低，尤其是在第一种应用方法中。所有这些结果表明，SA 对 PS II 和抗氧化防御系统的影响取决于所使用的浓度和使用方法。
To study the effects of application of salicylic acid (SA) on the antioxidant system and photosystem II (PS II) in wheat seedlings we used two different experiments. The first method was carried out by immersing roots in Hoagland's nutrient solution containing 0, 0.25, or 2.5 mM SA, and the second method was performed by spraying two-week-old seedlings with the same SA concentrations. After 24 h, chlorophyll fluorescence, thylakoid membrane proteins, antioxidant enzyme activities, and reactive oxygen species were measured. The low concentration of SA caused a significant increase in the antioxidant enzyme activities. However, the treatment with 2.5 mM SA resulted in an increase in the non-photochemical quenching coefficient and a decrease in the antioxidant enzyme activities, the quantum yield of PS II photochemistry, and the photochemical quenching, especially in the first method of application. All these results indicate that the effects of SA on PS II and the antioxidative defense system were dependent on the concentration used and the method of application.