Identification and characterization of the Tupaia herpesvirus DNA polymerase gene.
Identification and characterization of the Tupaia herpesvirus DNA polymerase gene.
复制标题
树鼩疱疹病毒 DNA 聚合酶基因的鉴定和表征。
作者：
C. Springfeld;C. Tidona;R. Kehm;U. Bahr;G. Darai
树鼩疱疹病毒（Tupaia herpesviruses，THV）已经从树鼩（Tupoia spp.）的恶性淋巴瘤和退化的肺或脾细胞培养物中分离出来，但是由于缺乏遗传信息，THV的最终分类仍然是开放的。在目前的工作中，病毒DNA聚合酶（DPOL）基因的基因组内的不同THV株使用PCR和简并寡核苷酸引物映射。测定THV毒株1至5的DPOL基因的核苷酸序列并用于比较分析。RT-PCR证实了THV-2 DPOL基因的转录活性。发现不同的THV毒株彼此非常密切相关。与其他疱疹病毒相比，检测到的最高氨基酸序列同一性是与鼠和人巨细胞病毒的DPOL。这些结果证明了THV是β疱疹病毒亚科成员的结论。
Tupaia herpesviruses (THVs) have been isolated from malignant lymphomas and from degenerating lung or spleen cell cultures of tree shrews (Tupoia spp.), but because of a lack of genetic information the final classification of THVs is still open. In the present work the viral DNA polymerase (DPOL) gene was mapped within the genome of the different THV strains using PCR and degenerate oligonucleotide primers. Nucleotide sequences of the DPOL genes of THV strains 1 to 5 were determined and used for comparative analyses. The transcriptional activity of the THV-2 DPOL gene was confirmed by RT-PCR. It was found that the different THV strains are very closely related to each other. When compared to other herpesviruses the highest amino acid sequence identities detected were with DPOLs of the murine and human cytomegaloviruses. These results justify the conclusion that THVs are members of the subfamily Betaherpes-virinae.
影响因子：
3.7
作者：
ELLIOTT, R;CLARK, C;SPECTOR, DH
通讯作者：
SPECTOR, DH