Evaluation of osteogenic inductivity of a novel BMP2-mimicking peptide P28 and P28-containing bone composite.
Evaluation of osteogenic inductivity of a novel BMP2-mimicking peptide P28 and P28-containing bone composite.
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DOI：
10.1002/jbm.a.36228
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Ting-fang Sun;Yanzhen Qu;Wei Cui;Liang Yang;Yanhui Ji;Wei-wei Yu;Ramphul Navinduth;Zengwu Shao;Hu Yang;Xiao-dong Guo
中科院分区：
文献类型：
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作者：
Ting-fang Sun;Yanzhen Qu;Wei Cui;Liang Yang;Yanhui Ji;Wei-wei Yu;Ramphul Navinduth;Zengwu Shao;Hu Yang;Xiao-dong Guo
我们开发了一种新的BMP 2模拟肽P28，它具有两个独特的特殊结构元件-七个重复的天冬氨酸和磷酸化丝氨酸，旨在促进对羟基磷灰石的亲和力以及磷灰石结晶和矿化的成核。在这项工作中，我们系统地研究了P28在体外和体内的成骨诱导性，并与BMP 2进行比较。我们证实了其对体外MC 3 T3-E1细胞增殖、募集和成骨分化的影响。此外，我们将P28负载到仿生骨复合材料纳米羟基磷灰石/胶原/聚（l-乳酸）（nHAC/PLA）中，并使用兔股骨髁缺损模型评价其促进骨再生的能力。显微CT重建定量分析和组织学分析表明，P28/nHAC/PLA骨复合材料具有修复骨缺损的能力。© 2017 Wiley Periodicals，Inc. J Biomed Mater Res Part A：106A：210-220，2018.
We developed a novel BMP2-mimicking peptide P28, which possesses two distinct special structural elements-seven repetitive aspartic acids and phosphorylated serine designed to promote affinity to hydroxyapatite as well as nucleation of apatite crystallization and mineralization. In this work, we systematically studied the osteogenic inductivity of P28 in vitro and in vivo in comparison with BMP2. We confirmed its effects on proliferation, recruitment, and osteogenic differentiation of MC3T3-E1 cells in vitro. Furthermore, we loaded P28 into a biomimetic bone composite nanohydroxyapatite/collagen/poly(l-lactide) (nHAC/PLA) and evaluated its ability to promote bone regeneration in vivo using a rabbit femoral condyle defect model. The micro-CT reconstruction and quantification analysis and the histological analysis indicated that P28/nHAC/PLA bone composite was capable of repairing bone defects. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 210-220, 2018.