Mass spectral comparison of the neuropeptide complement of the stomatogastric ganglion and brain in the adult and embryonic lobster, Homarus americanus
Mass spectral comparison of the neuropeptide complement of the stomatogastric ganglion and brain in the adult and embryonic lobster, Homarus americanus
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DOI：
10.1111/j.1471-4159.2007.05154.x
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发表时间：
2008-05-01
影响因子：
4.7
通讯作者：
Li, Lingjun
中科院分区：
文献类型：
--
作者：
Cape, Stephanie S.;Rehm, Kristina J.;Li, Lingjun
采用多方面质谱技术，对成年和胚胎晚期美洲梭子蟹的口胃神经节（STG）和大脑中的神经肽进行了比较。总体而言，29个神经肽从10个家庭被确定在大脑和/或龙虾的STG。这些神经肽中的许多是首次在胚胎龙虾中报道的。神经肽提取，然后通过液相色谱法耦合到四极杆飞行时间质谱法，使24个先前表征的肽在成人脑和13个肽在胚胎脑的信心鉴定。对两个新的肽（QDLDHVFLRFa和GPPSLRLRFa）进行从头测序。此外，成人胚胎脑的比较揭示了存在的不完全加工形式的北极光速激肽相关肽1a（CabTRP 1a，APSGFLGMRG）只在胚胎脑。成体与胚胎STG的比较显示，QDLDHVFLRFa存在于胚胎STG中，但在成体STG中不存在，CabTRP 1a表现出相反的趋势。在STG中的神经肽的相对定量显示，三个orcokinin家族肽（NFDEIDRSGFGF，NFDEIDRSGFGFV，和NFDEIDRSGFGFN），B型allatostatin（STNWSSLRSAWa），和orcomotropin相关肽（FDAFTTGFGHS）在成人相对于胚胎STG表现出更高的信号强度。RFamide（Arg-Phe-amide）家族肽（DTSTPALRLRFa）、[瓦尔（1）]SIFamide（VYRKPPFNGSIFa）和orcokinin相关肽（VYGPRDIANLY）在胚胎STG谱中比在成人STG谱中更强。总的来说，这项研究扩展了我们目前对H。americanus neuropeptidome，并强调了发育过程中发生的一些有趣的表达差异。
Neuropeptides in the stomatogastric ganglion (STG) and the brain of adult and late embryonic Homarus americanus were compared using a multi-faceted mass spectral strategy. Overall, 29 neuropeptides from 10 families were identified in the brain and/or the STG of the lobster. Many of these neuropeptides are reported for the first time in the embryonic lobster. Neuropeptide extraction followed by liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry enabled confident identification of 24 previously characterized peptides in the adult brain and 13 peptides in the embryonic brain. Two novel peptides (QDLDHVFLRFa and GPPSLRLRFa) were de novo sequenced. In addition, a comparison of adult to embryonic brains revealed the presence of an incompletely processed form of Cancer borealis tachykinin-related peptide 1a (CabTRP 1a, APSGFLGMRG) only in the embryonic brain. A comparison of adult to embryonic STGs revealed that QDLDHVFLRFa was present in the embryonic STG but absent in the adult STG, and CabTRP 1a exhibited the opposite trend. Relative quantification of neuropeptides in the STG revealed that three orcokinin family peptides (NFDEIDRSGFGF, NFDEIDRSGFGFV, and NFDEIDRSGFGFN), a B-type allatostatin (STNWSSLRSAWa), and an orcomyotropin-related peptide (FDAFTTGFGHS) exhibited higher signal intensities in the adult relative to the embryonic STG. RFamide (Arg-Phe-amide) family peptide (DTSTPALRLRFa), [Val(1)]SIFamide (VYRKPPFNGSIFa), and orcokinin-related peptide (VYGPRDIANLY) were more intense in the embryonic STG spectra than in the adult STG spectra. Collectively, this study expands our current knowledge of the H. americanus neuropeptidome and highlights some intriguing expression differences that occur during development.