Sampling of intestinal microbiota and targeted amplification of bacterial 16S rRNA genes for microbial ecologic analysis.
Sampling of intestinal microbiota and targeted amplification of bacterial 16S rRNA genes for microbial ecologic analysis.
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DOI：
10.1002/0471142735.im0741s107
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发表时间：
2014-11-03
影响因子：
--
通讯作者：
Braun, Jonathan
中科院分区：
文献类型：
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作者：
Tong, Maomeng;Jacobs, Jonathan P;McHardy, Ian H;Braun, Jonathan
宿主相关肠道微生物群的生态失调正在成为免疫、代谢和行为疾病的风险和表型的重要因素。适当收集和预处理来自人类或小鼠的生物标本对于准确分析微生物组成和功能状态是必要的。本文介绍了从人类和小鼠肠道腔和粘膜微生物群中取样，并使用16S rRNA测序分析微生物系统发育组成的方法。使用该方案生成的数据可用于下游微生物生态学的定量分析。
Dysbiosis of host-associated commensal microbiota is emerging as an important factor in risk and phenotype of immunologic, metabolic, and behavioral diseases. Appropriate collection and pre-processing of biospecimens from humans or mice is necessary for accurate analysis of microbial composition and functional state. Methods to sample intestinal luminal and mucosal microbiota from humans and mice, and to profile microbial phylogenetic composition using 16S rRNA sequencing are presented here. Data generated using this protocol can be used for downstream quantitative analysis of microbial ecology.